Limitations of dynamic contrast-enhanced MRI in monitoring radiation-induced changes in the fraction of radiobiologically hypoxic cells in human melanoma xenografts
| Autor: | Ilana C. Benjaminsen, Berit Mathiesen, Einar K. Rofstad, Elin A. Melås |
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| Rok vydání: | 2008 |
| Předmět: |
Gadolinium DTPA
Contrast Media Mice Nude Radiation induced Fraction (chemistry) Radiation Dosage Mice Cell Line Tumor Medicine Animals Humans Radiology Nuclear Medicine and imaging Irradiation skin and connective tissue diseases Melanoma Mice Inbred BALB C Tumor hypoxia medicine.diagnostic_test business.industry Magnetic resonance imaging Dose-Response Relationship Radiation Magnetic Resonance Imaging Cell Hypoxia Dynamic contrast-enhanced MRI Human melanoma Female Dose Fractionation Radiation Nuclear medicine business Perfusion |
| Zdroj: | Journal of magnetic resonance imaging : JMRI. 28(5) |
| ISSN: | 1053-1807 |
| Popis: | Purpose To investigate the potential of gadopentetate dimeglumine (Gd-DTPA)-based dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in detecting radiation-induced changes in the fraction of radiobiologically hypoxic cells in A-07 human melanoma xenografts. Materials and Methods A-07 tumors were randomly assigned to an unirradiated control group or a group given a single radiation dose of 20 Gy. DCE-MRI and measurement of fraction of hypoxic cells were performed immediately before and 24 h after the radiation exposure. Tumor images of E · F (E is the initial extraction fraction of Gd-DTPA and F is blood perfusion) and λ (λ is proportional to extracellular volume fraction) were produced by subjecting DCE-MRI series to Kety analysis. Fraction of hypoxic cells was measured by using a radiobiological assay based on the paired survival curve method. Results Fraction of radiobiologically hypoxic cells was higher in irradiated tumors (26.2 ± 5.8%) than in unirradiated tumors (7.5 ± 2.7%) by a factor of 3.5 ± 1.5 (P = 0.0093), whereas only minor radiation-induced changes in E · F and λ could be detected. Conclusion DCE-MRI does not seem to offer insight into the changes in fraction of radiobiologically hypoxic cells occurring in A-07 tumors within 24 h after irradiation with 20 Gy. J. Magn. Reson. Imaging 2008;28:1209–1218. © 2008 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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