ROS Scavenger Decreases Basal Perfusion Pressure, Vasoconstriction and NO Synthase Activity in Pulmonary Circulation During Pulmonary Microembolism
| Autor: | Roman Mizera, Jan Herget, Daniel Hodyc |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Pulmonary Circulation medicine.medical_specialty Physiology Blood Pressure Oxidative phosphorylation Cyclic N-Oxides Organ Culture Techniques Internal medicine medicine Animals Rats Wistar chemistry.chemical_classification Reactive oxygen species Microcirculation Free Radical Scavengers General Medicine Hypoxia (medical) medicine.disease Pulmonary hypertension Rats Pulmonary embolism Enzyme Activation NG-Nitroarginine Methyl Ester Endocrinology chemistry Vasoconstriction Anesthesia cardiovascular system Spin Labels Sodium nitroprusside Nitric Oxide Synthase medicine.symptom Pulmonary Embolism Reactive Oxygen Species Perfusion circulatory and respiratory physiology medicine.drug |
| Zdroj: | Physiological Research. :683-688 |
| ISSN: | 1802-9973 0862-8408 |
| DOI: | 10.33549/physiolres.932906 |
| Popis: | Two mechanisms contribute in the development of pulmonary hypertension in pulmonary embolism (PE) – obstruction of pulmonary blood vessels and vasoconstriction. We hypothesize that hypoxia, increased shear stress and/or activation of gathered leukocytes in the PE may cause a release of reactive oxygen species (ROS). Therefore our aim was to determine the influence of the ROS scavenger Tempol on pulmonary hypertension and to describe NO synthase activity and production of NO oxidative products (NOx) after PE. In general anesthesia sephadex microspheres suspended in PSS were applied in right jugular vein as the pulmonary microembolism. Than we measured in isolated salt solution-perfused lungs the changes in perfusion pressure, activity of NO synthase and NOx plasma concentration in 7 groups of rats: C: control group (n=5), CN: C + sodium nitroprusside (SN) (n=5), EN: PE + SN (n=5), ETN: Tempol + PE + SN (n=5), CL: C + L-NAME (n=5), EL: PE + L-NAME (n=5), ETL: Tempol + PE + L-NAME (n=5). Tempol was applied intraperitoneally before PE. Animals that received Tempol (groups TN, TL) had significantly lower basal perfusion pressure than those which did not receive Tempol (EN, EL). Overall we measured a higher decrease of perfusion pressure than in the control group (C) after application of SN. Administration of L NAME after PE (EL) increased the pressure more than in the control group (NL). NOx concentration was higher after PE. We found that preventive administration of Tempol decreases the increase in perfusion pressure after PE. PE increased NO release and concentration of NOx. |
| Databáze: | OpenAIRE |
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