Key genes expressed in different stages of spinal cord ischemia/reperfusion injury
| Autor: | Zhigang Liu, Changjun Zheng, Zhiping Qi, Xiaoyu Yang, Chun-fang Zan, Ting-ting Hou, Chun-xu Li, Peng Xia, Jian-an Li |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty mRNA Ischemia microRNA arrays ischemia/reperfusion injury lcsh:RC346-429 Tmem69 03 medical and health sciences 0302 clinical medicine Cxcl10 Developmental Neuroscience medicine.artery microRNA medicine nerve regeneration Spinal cord injury lcsh:Neurology. Diseases of the nervous system Aorta business.industry Abdominal aorta bioinformatics medicine.disease Spinal cord spinal cord injury 030104 developmental biology medicine.anatomical_structure transcriptome neural regeneration RNA extraction business Reperfusion injury 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research, Vol 11, Iss 11, Pp 1824-1829 (2016) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.194754 |
| Popis: | The temporal expression of microRNA after spinal cord ischemia/reperfusion injury is not yet fully understood. In the present study, we established a model of spinal cord ischemia in Sprague-Dawley rats by clamping the abdominal aorta for 90 minutes, before allowing reperfusion for 24 or 48 hours. A sham-operated group underwent surgery but the aorta was not clamped. The damaged spinal cord was removed for hematoxylin-eosin staining and RNA extraction. Neuronal degeneration and tissue edema were the most severe in the 24-hour reperfusion group, and milder in the 48-hour reperfusion group. RNA amplification, labeling, and hybridization were used to obtain the microRNA expression profiles of each group. Bioinformatics analysis confirmed four differentially expressed microRNAs (miR-22-3p, miR-743b-3p, miR-201-5p and miR-144-5p) and their common target genes (Tmem69 and Cxcl10). Compared with the sham group, miR-22-3p was continuously upregulated in all three ischemia groups but was highest in the group with no reperfusion, whereas miR-743b-3p, miR-201-5p and miR-144-5p were downregulated in the three ischemia groups. We have successfully identified the key genes expressed at different stages of spinal cord ischemia/reperfusion injury, which provide a reference for future investigations into the mechanism of spinal cord injury. |
| Databáze: | OpenAIRE |
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