Short-Chained Oligo(ethylene oxide)-Functionalized Gold Nanoparticles: Realization of Significant Protein Resistance
| Autor: | David J. Vanderah, Marlon L. Walker, Kathryn R. Riley, Christopher M. Sims, Imani T. Wood |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Ethylene Oxide
Models Molecular Surface Properties Metal Nanoparticles Protein Corona 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Article Citric Acid Analytical Chemistry Nanomaterials Capillary electrophoresis Dynamic light scattering Zeta potential Organic chemistry Animals Sulfhydryl Compounds Chemistry Electrophoresis Capillary Fibrinogen 021001 nanoscience & nanotechnology Binding constant Dynamic Light Scattering 0104 chemical sciences Chemical engineering Colloidal gold Cattle Muramidase Adsorption Gold 0210 nano-technology Chickens Protein adsorption |
| Popis: | Protein corona formed on nanomaterial surfaces play an important role in the bioavailability and cellular uptake of nanomaterials. Modification of surfaces with oligoethylene glycols (OEG) are a common way to improve the resistivity of nanomaterials to protein adsorption. Short-chain ethylene oxide (EO) oligomers have been shown to improve the protein resistance of planar Au surfaces. We describe the application of these EO oligomers for improved protein resistance of 30 nm spherical gold nanoparticles (AuNPs). Functionalized AuNPs were characterized using UV-Vis spectroscopy, dynamic light scattering (DLS), and zeta potential measurements. Capillary electrophoresis (CE) was used for separation and quantitation of AuNPs and AuNP-protein mixtures. Specifically, nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) was employed for the determination of equilibrium and rate constants for binding between citrate-stabilized AuNPs and two model proteins, lysozyme and fibrinogen. Semi-quantitative CE analysis was carried out for mixtures of EO-functionalized AuNPs and proteins, and results demonstrated a 2.5-fold to 10-fold increase in protein binding resistance to lysozyme depending on the AuNP surface functionalization and a 15-fold increase in protein binding resistance to fibrinogen for both EO oligomers examined in this study. Graphical abstract Using capillary electrophoresis, the addition of short-chained oligo(ethylene oxide) ligands to gold nanoparticles was shown to improve protein binding resistance up to 15-fold. |
| Databáze: | OpenAIRE |
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