Ghrelin-O-Acyltransferase (GOAT) Enzyme as a Novel Potential Biomarker in Gastroenteropancreatic Neuroendocrine Tumors
| Autor: | Manuel D. Gahete, Rafael Sánchez-Sánchez, María A Gálvez-Moreno, Antonio J. Martínez-Fuentes, Sergio Pedraza-Arevalo, Raquel Serrano-Blanch, Emilia Alors-Perez, Justo P. Castaño, Raúl M. Luque, Aura D. Herrera-Martínez |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Neuroendocrine tumors Article 03 medical and health sciences 0302 clinical medicine Predictive Value of Tests Stomach Neoplasms Internal medicine Cell Line Tumor Intestinal Neoplasms Biomarkers Tumor Medicine Humans Receptor Receptors Ghrelin Aged Cell Proliferation Neoplasm Staging chemistry.chemical_classification business.industry Cell growth digestive oral and skin physiology Gastroenterology Middle Aged medicine.disease Ghrelin O-acyltransferase Ghrelin Enzymes Pancreatic Neoplasms Neuroendocrine Tumors 030104 developmental biology Enzyme Endocrinology chemistry Cell culture 030220 oncology & carcinogenesis Case-Control Studies Disease Progression Immunohistochemistry Female Neoplasm Grading business Acyltransferases Biomarkers |
| Zdroj: | Clinical and Translational Gastroenterology 9(10):196 (2018) Helvia. Repositorio Institucional de la Universidad de Córdoba instname Clinical and Translational Gastroenterology |
| Popis: | Objectives: The association between the presence and alterations of the components of the ghrelin system and the development and progression of neuroendocrine tumors (NETs) is still controversial and remains unclear. Methods: Here, we systematically evaluated the expression levels (by quantitative-PCR) of key ghrelin system components of in gastroenteropancreatic (GEP)-NETs, as compared to non-tumor adjacent (NTA; n = 42) and normal tissues (NT; n = 14). Then, we analyzed their putative associations with clinical-histological characteristics. Results: The results indicate that ghrelin and its receptor GHSR1a are present in a high proportion of normal tissues, while the enzyme ghrelin-O-acyltransferase (GOAT) and the splicing variants In1-ghrelin and GHSR1b were present in a lower proportion of normal tissues. In contrast, all ghrelin system components were present in a high proportion of tumor and NTA tissues. GOAT was significantly overexpressed (by quantitative-PCR (qPCR)) in tumor samples compared to NTA, while a trend was found for ghrelin, In1-ghrelin and GHSR1a. In addition, expression of these components displayed significant correlations with key clinical parameters. The marked overexpression of GOAT in tumor samples compared to NTA regions was confirmed by IHC, revealing that this enzyme is particularly overexpressed in gastrointestinal NETs, where it is directly correlated with tumor diameter. Conclusions: These results provide novel information on the presence and potential pathophysiological implications of the ghrelin system components in GEP-NETs, wherein GOAT might represent a novel diagnostic biomarker. |
| Databáze: | OpenAIRE |
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