Pharmacological activities of the organic extracts and fatty acid composition of the petroleum ether extract from Haplophyllum tuberculatum leaves
| Autor: | Yvan Vander Heyden, Assia Hamdi, Amal Abdelhamid, Abderrahman Bouraoui, Hèla Belghith, Imed Chraief, Kaouther Majouli, Belsem Marzouk, Zohra Marzouk |
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| Přispěvatelé: | Analytical Chemistry and Pharmaceutical Technology, Pharmaceutical and Pharmacological Sciences, Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Hot Temperature Anti-Inflammatory Agents Ethyl acetate Carrageenan Palmitic acid Mice chemistry.chemical_compound 0302 clinical medicine Drug Discovery Edema Petroleum ether Acetic Acid Organic extracts Analgesics Traditional medicine Fatty Acids Phytochemical Erucic acid 030220 oncology & carcinogenesis Female Stearic acid Linoleic acid Pain Gas Chromatography-Mass Spectrometry Lethal Dose 50 03 medical and health sciences Haplophyllum tuberculatum Alkanes Animals Stomach Ulcer Rats Wistar gamma-Linolenic Acid Rutaceae Plants Medicinal Dose-Response Relationship Drug Ethanol Plant Extracts Anti-Ulcer Agents Plant Leaves Disease Models Animal Oleic acid 030104 developmental biology Pharmacological activities chemistry Solvents Hydrochloric Acid pharmacology Phytotherapy |
| DOI: | 10.1016/j.jep.2018.01.012 |
| Popis: | Ethnopharmacological relevance Haplophyllum tuberculatum is used in traditional medicine to treat many disorders including inflammation and pain. The aim of this study is to investigate the organic extracts from H. tuberculatum leaves against inflammation, gastric ulcer and pain. Materials and methods: Acute toxicity was studied in vivo to determine the toxic doses of the organic extracts. Anti-inflammatory activity was also evaluated in vivo using carrageenan-induced paw edema in Wistar rats. Gastroprotective activity was tested using the HCl/ethanol-induced gastric ulcer test in rats. Peripheral and central analgesic activities were assessed using the acetic acid-induced writhing test and the hot-plate method, respectively. The chemical composition of the fatty acids in the petroleum ether (PE) extract was determined with GC-MS. Results At 25, 50 and 100 mg/kg PE extract was the most active against inflammation. Percentages inhibition 5 h after carrageenan-injection were 51.12; 86.71% and 96.92%, respectively. The same extract at 100 mg/kg showed good analgesic activities using the acetic acid-induced writhing test and the hot-plate method. The chloroform, ethyl acetate (EtOAc) and butanolic (n-BuOH) extracts exhibited strong anti-inflammatory, gastroprotective and analgesic activities at 100 mg/kg. The GC-FID analysis revealed that the PE extract was rich in γ-linolenic acid (45.50%) followed by palmitic acid (18.48%), linoleic acid (10.73%), erucic acid (4.72), stearic acid (3.96%) and oleic acid (2.57%). Conclusion The results of the present study support the traditional use of the leaves of H. tuberculatum and may possibly serve as prospective material for further development of safe new phytochemical anti-inflammatory, gastroprotective and/or analgesic agents. |
| Databáze: | OpenAIRE |
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