Independence of buoyant cell density and growth rate in Escherichia coli

Autor:	S J Schroeter, R Graetzer, W W Baldwin, H E Kubitschek
Rok vydání:	1984
Předmět:	Cell division Temperature Buoyant density Biology medicine.disease_cause Microbiology Kinetics Cell density Centrifugation Density Gradient Escherichia coli medicine Biophysics Centrifugation Growth rate Equilibrium Centrifugation Molecular Biology Percoll Research Article
Zdroj:	Journal of Bacteriology. 158:296-299
ISSN:	1098-5530 0021-9193
Popis:	The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth rate in any of three sets of experiments in which different gradient conditions were used. In addition, when cultures were allowed to enter the stationary phase of growth, mean cell volumes and buoyant densities usually remained unchanged for extended periods. These and earlier results support the existence of a highly regulated, discrete state of buoyant density during steady-state growth of E. coli and other cells that divide by equatorial fission.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff0b35f5ae6d846bb0436036701ffdc8 https://doi.org/10.1128/jb.158.1.296-299.1984 Zobrazit plný text záznamu