Association Between Single Nucleotide Polymorphisms in the Cyclooxygenase-2, Tumor Necrosis Factor-α, and Vascular Endothelial Growth Factor-A Genes, and Susceptibility to Hepatocellular Carcinoma
| Autor: | Giuseppe Montalto, Maurizio Soresi, Terranova A, Giacalone A, Marasa' L, Daniele Balasus, Lydia Giannitrapani, Melchiorre Cervello |
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| Přispěvatelé: | Giacalone, A, Montalto, G, Giannitrapani, L, Balasus, D, Terranova, A, Cervello, M, Soresi, M, Marasà, L. |
| Rok vydání: | 2011 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Heterozygote Carcinoma Hepatocellular Cirrhosis Angiogenesis Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Biochemistry COX-2 TNFa VEGF Hepatocellular Carcinoma SNPs Genetics medicine Humans neoplasms Molecular Biology Tumor Necrosis Factor-alpha Homozygote Liver Neoplasms nucleotide polymorphisms cyclooxygenase-2 tumor necrosis factor-α vascular endothelial growth factor-A genes hepatocellular carcinoma Heterozygote advantage medicine.disease digestive system diseases Vascular endothelial growth factor A Cyclooxygenase 2 Hepatocellular carcinoma Immunology Cancer research Molecular Medicine Female Tumor necrosis factor alpha Restriction fragment length polymorphism Biotechnology |
| Zdroj: | OMICS: A Journal of Integrative Biology. 15:193-196 |
| ISSN: | 1557-8100 1536-2310 |
| DOI: | 10.1089/omi.2010.0095 |
| Popis: | Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-α (TNF-α) are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellular carcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms (SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore the proteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agents have entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-α, and VEGF-A genes in patients with HCC versus LC patients and a control group. The aim of this article was to verify the correlation between the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162 healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLP-PCR) method. The SNPs analyzed were: -1195 GA of the COX-2 gene, -308 GA of the TNF-α gene, and +936 CT of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our results confirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC (p=0.039), suggesting that this SNP may predispose to the development of HCC. |
| Databáze: | OpenAIRE |
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