In-Host Emergence of Linezolid Resistance in a Complex Pattern of Toxic Shock Syndrome Toxin-1-Positive Methicillin-Resistant Staphylococcus aureus Colonization in Siblings with Cystic Fibrosis
| Autor: | Jean-Philippe Lavigne, Alexandre Jay, Hélène Marchandin, Agathe Boudet, Raphaël Chiron, Catherine Dunyach-Remy |
|---|---|
| Přispěvatelé: | Virulence bactérienne et maladies infectieuses (VBMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Centre Hospitalier Universitaire de Nîmes (CHU Nîmes), Hydrosciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Salvy-Córdoba, Nathalie |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis Antibiotics Resistance Toxic shock syndrome toxin 1 Staphylococcus aureus Toxicology medicine.disease_cause [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract chemistry.chemical_compound [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Child 0303 health sciences education.field_of_study Diversity MESH: Microbial Sensitivity Tests Geraldine clone MESH: Multilocus Sequence Typing [SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Medicine MESH: Whole Genome Sequencing MESH: Cystic Fibrosis medicine.drug_class Population MESH: Shock Septic MESH: Linezolid MESH: Staphylococcal Infections Single-nucleotide polymorphism Emergence MESH: Methicillin-Resistant Staphylococcus aureus Biology Cystic fibrosis Microbiology 03 medical and health sciences 23S ribosomal RNA MESH: Genotyping Techniques MESH: Drug Resistance Bacterial medicine Adaptation education 030304 developmental biology MESH: Adolescent MESH: Humans 030306 microbiology Siblings Linezolid Toxic shock syndrome MESH: Drug Resistance Multiple Bacterial biochemical phenomena metabolism and nutrition medicine.disease bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Siblings chemistry [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Female |
| Zdroj: | Toxins Toxins, 2021, 13 (5), pp.317. ⟨10.3390/toxins13050317⟩ Toxins, Vol 13, Iss 317, p 317 (2021) Volume 13 Issue 5 |
| ISSN: | 2072-6651 |
| Popis: | International audience; Methicillin-resistant Staphylococcus aureus (MRSA) can cause chronic lung infections in patients with Cystic Fibrosis (CF). One option for managing them is the use of linezolid. We hereby report the in-host emergence of linezolid resistance (LR) in MRSA in CF siblings via a population analysis. A collection of 171 MRSA strains from 68 samples were characterized by determining their linezolid Minimal Inhibitory Concentrations (MICs), analyzing the locus of staphylococcal protein A (spa) and whole genome sequencing. Courses of linezolid were retraced. Strains belonged to three spa types (t002, t045, t127) and two sequence types (ST1, ST5). Emergence of LR occurred under treatment, one year apart in both siblings, in the CC5-MRSA-I Geraldine clone harboring the toxic shock syndrome toxin-1-encoding gene. Resistance was related to a G2576T substitution present in a variable number of 23S rRNA gene copies. Susceptible and resistant strains were co-isolated within samples. Single Nucleotide Polymorphism-based analysis revealed complex colonizations by highly diversified, clonally related populations. LR remains rare in MRSA and there are very few longitudinal analyses documenting its emergence. Analyzing a large MRSA collection revealed new aspects of LR emergence: it emerges in specific subclonal lineages resulting from adaptive diversification of MRSA in the CF lung and this heterogeneity of intra-sample resistance may contribute to compromising antibiotic management. |
| Databáze: | OpenAIRE |
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