Cellular and Molecular Characteristics of Transformed T Cells from an Antigen-Specific T-Cell Line
| Autor: | B. Holm, Carsten Geisler, Bent Rubin, A.-M. K. Wegener |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
T-Lymphocytes
T cell Immunology Immunoglobulin Variable Region Receptors Antigen T-Cell Mice Inbred Strains chemical and pharmacologic phenomena Major histocompatibility complex Epitope Epitopes Mice Antigens Neoplasm hemic and lymphatic diseases medicine Animals RNA Neoplasm Beta (finance) Cell Line Transformed biology T-cell receptor Antibodies Monoclonal DNA Neoplasm General Medicine T lymphocyte Blotting Northern Molecular biology Blotting Southern Cell Transformation Neoplastic Phenotype medicine.anatomical_structure Cell culture Monoclonal biology.protein DNA Probes Neoplasm Transplantation |
| Zdroj: | University of Copenhagen |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1111/j.1365-3083.1990.tb02815.x |
| Popis: | An antigen-specific T-cell line which transforms into T-lymphoma cells in vitro but apparently not in vivo is described. Membrane markers, tumorigenicity and T-cell receptor (TcR) V alpha and V beta-gene usage of the in vitro transformed T-cell line were analysed to investigate whether the transformation event was poly-, oligo-, or monoclonal. The results indicate that the T lymphoma has no chromosome abnormalities, contains no tumour-inducing virus, can induce clone-specific immunity, and is oligoclonal with respect to TcR V alpha and V beta expression. The nature of the transformation event and clinical application of vaccination against T lymphomas is discussed. In addition, the expressed TcR V alpha and V beta repertoire of Con A T blasts was apparently not affected by the Igh-l or the MHC haplotype, as investigated in Igh-l and MHC congeneic C57Bl mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text