Interaction of Concanavalin A with the Cell Wall of Bacillus subtilis
Autor: | D. C. Birdsell, Ronald J. Doyle |
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Rok vydání: | 1972 |
Předmět: |
Immunodiffusion
Autolysis (biology) Physiology and Metabolism chemical and pharmacologic phenomena Bacillus subtilis Sodium Chloride Biology Polysaccharide Vibration Microbiology Bacterial cell structure Potassium Chloride Cell wall chemistry.chemical_compound Cell Wall Lectins Concanavalin A Chemical Precipitation Ultrasonics Trichloroacetic Acid Trichloroacetic acid Molecular Biology chemistry.chemical_classification Teichoic acid Binding Sites Viscosity Sodium Hydrogen-Ion Concentration Iodides biology.organism_classification Culture Media Teichoic Acids Solubility chemistry Biochemistry biology.protein Muramidase Autolysis Glycogen Densitometry |
Zdroj: | Journal of Bacteriology. 109:652-658 |
ISSN: | 1098-5530 0021-9193 |
Popis: | Interactions between concanavalin A and cell wall digests of Bacillus subtilis 168 resulted in insoluble complexes as observed by double gel diffusion, turbidity, and analysis of the precipitate. The macromolecular constituent of the cell walls complexing with concanavalin A was the polyglucosylglycerol phosphate teichoic acid. The complex exhibited two p H optima: 3.1 and 7.4. The complex could be dissociated by saccharides which bind to concanavalin A. In contrast to concanavalin A-neutral polysaccharide complexes, formation of the concanavalin A-wall complex was inhibited by salts. It was subsequently shown that salts induce conformational changes in cell wall digests. The data suggested that for complex formation to occur a rigid rod conformation in the glucosylated teichoic acid is probably necessary. Concanavalin A can be used as a probe to study structural features of bacterial cell walls. |
Databáze: | OpenAIRE |
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