Actin-Binding Cellular Proteins inside Human Immunodeficiency Virus Type 1

Autor: Young D. Kim, Xiao Zhen Zhou, Raymond C. Sowder, Kun Ping Lu, Lori V. Coren, David E. Ott, Louis E. Henderson, Robert J. Fisher, Donald G. Johnson, Bradley P. Kane
Rok vydání: 2000
Předmět:
medicine.medical_treatment
Immunoblotting
Molecular Sequence Data
Phosphatidylethanolamine Binding Protein
Androgen-Binding Protein
03 medical and health sciences
Non-histone protein
Peptide Elongation Factor 1
HIV Protease
Sequence Analysis
Protein
Virology
medicine
Humans
Actin-binding protein
Amino Acid Sequence
Phospholipid Transfer Proteins
Peptide sequence
Polyacrylamide gel electrophoresis
Actin
Chromatography
High Pressure Liquid
030304 developmental biology
Adaptor Proteins
Signal Transducing
Monomeric GTP-Binding Proteins
0303 health sciences
Protease
biology
030302 biochemistry & molecular biology
Microfilament Proteins
Subtilisin
Virion
Signal transducing adaptor protein
Glyceraldehyde-3-Phosphate Dehydrogenases
Blood Proteins
Chaperonin 60
NM23 Nucleoside Diphosphate Kinases
Peptidylprolyl Isomerase
Actins
3. Good health
Cell biology
NIMA-Interacting Peptidylprolyl Isomerase
Membrane protein
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Nucleoside-Diphosphate Kinase
biology.protein
HIV-1
Electrophoresis
Polyacrylamide Gel
Carrier Proteins
Transcription Factors
Zdroj: Virology. 266(1):42-51
ISSN: 0042-6822
DOI: 10.1006/viro.1999.0075
Popis: Host proteins are incorporated both on and inside human immunodeficiency virus type 1 (HIV-1) virions. To identify cellular proteins inside HIV-1, virion preparations were treated by a protease-digestion technique that removes external host proteins, allowing for the study of the proteins inside the virus. Treated HIV-1 preparations were analyzed by immunoblot, high-pressure liquid chromatography, and protein sequence analyses. These analyses identified several cellular proteins inside HIV-1: elongation factor 1α, glyceraldehyde-3-phosphate dehydrogenase, HS-1, phosphatidylethanolamine-binding protein, Pin1, Lck, Nm23-H1, and the C-terminal tail of CD43. Several of these proteins were found as fragments of their full-sized proteins that appear to be generated by our protease treatment of the virions, the HIV-1 protease, or a cellular protease. Recent advances in cell biology and biochemistry have identified some of these proteins as actin-binding proteins. These results support the hypothesis that actin filaments are incorporated into the virion and may provide additional clues for the understanding of the interaction between viral and cellular proteins during assembly and budding.
Databáze: OpenAIRE
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