Cross-infection of virulent Dichelobacter nodosus between sheep and co-grazing cattle
| Autor: | Hannah J. Jørgensen, Maren Knappe-Poindecker, Marianne Gilhuus, Synnøve Vatn, Tim Kåre Jensen, T. Fjeldaas |
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| Jazyk: | angličtina |
| Předmět: |
Cross infection
Veterinary medicine ved/biology.organism_classification_rank.species Cattle Diseases Sheep Diseases Virulence Cross-infection Dichelobacter nodosus Biology Polymerase Chain Reaction Microbiology Fusobacterium necrophorum Grazing medicine Pulsed-field gel electrophoresis Animals Treponema Foot Rot Sheep Treponemal Infections General Veterinary ved/biology General Medicine medicine.disease biology.organism_classification veterinary(all) Electrophoresis Gel Pulsed-Field Fusobacterium Infections Cattle Interdigital dermatitis Gram-Negative Bacterial Infections |
| Zdroj: | Knappe-Poindecker, M, Gilhuus, M, Jensen, T K, Vatn, S, Jørgensen, H J & Fjeldaas, T 2014, ' Cross-infection of virulent Dichelobacter nodosus between sheep and co-grazing cattle ', Veterinary Microbiology, vol. 170, no. 3-4, pp. 375-382 . https://doi.org/10.1016/j.vetmic.2014.02.044 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2014.02.044 |
| Popis: | Dichelobacter nodosus is the main aetiological agent of ovine footrot and the bacterium has also been associated with interdigital dermatitis is cattle. The aim of this study was to investigate possible cross-infection of virulent D. nodosus between sheep and co-grazing cattle. Five farms, where sheep previously diagnosed with virulent D. nodosus were co-grazing with cattle for different periods of time, were included. The study sample consisted of 200 cows and 50 sheep. All cows were examined for the presence of interdigital dermatitis, and ten ewes, preferably with symptoms of footrot, had the footrot scores recorded. On each farm, the same ten ewes and ten cows were chosen for bacterial analyses. Swabs were analysed for D. nodosus by PCR and culturing. D. nodosus isolates were virulence-tested and assigned to serogroups by fimA variant determination. Biopsies were evaluated histopathologically and analysed by fluorescent in situ hybridization for D. nodosus, Treponema spp. and Fusobacterium necrophorum. D. nodosus defined as virulent by the gelatin gel test were isolated from 16 sheep from four farms and from five cows from two of the same farms. All five cows had interdigital dermatitis. Two of the cows stayed infected for at least eight months. By pulsed-field gel electrophoresis (PFGE), the isolates from the five cows were found to be genetically indistinguishable or closely related to isolates from sheep from the same farm. This indicates that cross-infection between sheep and cows have occurred. |
| Databáze: | OpenAIRE |
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