Macrophage Responses to Silica Nanoparticles are Highly Conserved Across Particle Sizes
| Autor: | Norman J. Karin, Joel G. Pounds, Justin G. Teeguarden, Richard C. Zangar, Lisa M. Masiello, Brian D. Thrall, Barbara J. Tarasevich, Somnath Bandyopadhyay, Ryan D. Quesenberry, Katrina M. Waters |
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| Rok vydání: | 2008 |
| Předmět: |
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Silicon dioxide Nanoparticle Enzyme-Linked Immunosorbent Assay Biology Toxicology Cell Line Mice chemistry.chemical_compound Suspensions Gene expression Electrochemistry Animals Scattering Radiation RNA Messenger Particle Size Oligonucleotide Array Sequence Analysis Regulation of gene expression Systems Toxicology Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Macrophages Biological activity Silicon Dioxide Gene expression profiling Biochemistry chemistry Biophysics Nanoparticles Particle Particle size |
| Zdroj: | Toxicological Sciences. 107:553-569 |
| ISSN: | 1096-0929 1096-6080 |
| DOI: | 10.1093/toxsci/kfn250 |
| Popis: | Concerns about the potential adverse health effects of engineered nanoparticles stems in part from the possibility that some materials display unique chemical and physical properties at nanoscales which could exacerbate their biological activity. However, studies that have assessed the effect of particle size across a comprehensive set of biological responses have not been reported. Using a macrophage cell model, we demonstrate that the ability of unopsonized amorphous silica particles to stimulate inflammatory protein secretion and induce macrophage cytotoxicity scales closely with the total administered particle surface area across a wide range of particle diameters (7-500 nm). Whole genome microarray analysis of the early gene expression changes induced by 10- and 500-nm particles showed that the magnitude of change for the majority of genes affected correlated more tightly with particle surface area than either particle mass or number. Gene expression changes that were particle size-specific were also identified. However, the overall biological processes represented by all gene expression changes were nearly identical, irrespective of particle diameter. Direct comparison of the cell processes represented in the 10- and 500-nm particle gene sets using gene set enrichment analysis revealed that among 1009 total biological processes, none were statistically enriched in one particle size group over the other. The key mechanisms involved in silica nanoparticle-mediated gene regulation and cytotoxicity have yet to be established. However, our results suggest that on an equivalent nominal surface area basis, common biological modes of action are expected for nano- and supranano-sized silica particles. |
| Databáze: | OpenAIRE |
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