Polysaccharide from Hizikia Fusiformis Enhances the Immunomodulatory Activity of Macrophages
| Autor: | Choi Moon Hee, Hyun-Jae Shin, Jung Heon Lee, Eun-su Jang, Jieun Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
nf-kappa b polysaccharides lcsh:Medicine Inflammation Polysaccharide Microbiology 03 medical and health sciences 0302 clinical medicine Immunity sargassum Medicine chemistry.chemical_classification biology business.industry lcsh:R NFKB1 biology.organism_classification lcsh:Otorhinolaryngology immunity lcsh:RF1-547 030104 developmental biology chemistry inflammation 030220 oncology & carcinogenesis Sargassum Hizikia fusiformis medicine.symptom business |
| Zdroj: | Journal of Rhinology, Vol 27, Iss 1, Pp 8-15 (2020) |
| ISSN: | 2384-4361 1229-1498 |
| Popis: | Background and Objectives: Hizikia fusiformis is widely used in oriental health food in Japan, China, and Korea, and is known for its anti-oxidation properties.Materials and Method: In this study, we investigated the anti-inflammatory and immune-modulatory effects and mechanisms of Hizikia fusiformis (H. fusiformis) extracts in lipopolysaccharide (LPS)-treated RAW 264.7 cells. RAW 264.7 cells were incubated in the presence of different concentrations of the viscozyme component of H. fusiformis (1, 2, 5, and 10 μg/mL), and changes in expression of pro-inflammatory cytokines (GM-CSF, iNOS, VEGF, and COX-2) were evaluated by real-time PCR and immunoblotting. In addition, the associated signaling pathway including phospho (p)-pNF-κB 65, p-pIkBa, p-p38, and p-p44/42 was also evaluated.Results: The viscozyme component of H. fusiformis downregulated the expression of GM-CSF, iNOS, VEGF, and COX-2 mRNA. The augmented NO and ROS production was decreased by administration of H. fusiformis. The signal intensity of p-pNF-κB 65, p-pIkBa, p-p38, and p-p44/42 protein activated by LPS was ameliorated by administration of the viscozyme fraction in RAW 264.7 cells.Conclusion: These results suggest that H. fusiformis has potential as a therapeutic agent for inflammatory diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|