Simultaneous HPLC determination of metabolites of the inflammatory cascade
| Autor: | W. Diembeck, Andreas Schepky, R. Siegner |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Swine Lipoxygenase Immunology Prostaglandin Dermatitis Arachidonate 12-Lipoxygenase High-performance liquid chromatography Phospholipases A chemistry.chemical_compound Phospholipase A2 In vivo Animals Prostaglandins H 12-Hydroxy-5 8 10 14-eicosatetraenoic Acid Chromatography High Pressure Liquid Phospholipids Pharmacology chemistry.chemical_classification Arachidonic Acid Sheep Chromatography ATP synthase biology In vitro Phospholipases A2 Enzyme chemistry Biochemistry Prostaglandin-Endoperoxide Synthases biology.protein Prostaglandin H2 Arachidonic acid |
| Zdroj: | Inflammation Research. 46:417-419 |
| ISSN: | 1420-908X 1023-3830 |
| DOI: | 10.1007/s000110050214 |
| Popis: | Objective: A model approach is presented to the in vivo inflammation cascade, in which the activities of key enzymes (phospholipase A2 [3.1.1.4], prostaglandin synthase [EC 1.14.99.1], and lipoxygenases [EC 1.13.11.12]) are determined simultaneously in a single in vitro assay.¶Methods and Results: Detection of phospholipids (up to 50 pM) and arachidonic acid (up to 33 pM) is attained with high sensitivity and without radiolabelling using a SEDERE light scattering detector.¶Conclusions: Thus, in combination with a diode array UV-detector, lipids, prostaglandins, HETEs and other metabolites of the inflammation cascade can be determined with high efficiency using a reversed phase-high performance liquid chromatograph equipped with two highly sensitive detectors in series. |
| Databáze: | OpenAIRE |
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