Effects of cationic liposome-encapsulated doxycycline on experimental Chlamydia trachomatis genital infection in mice
| Autor: | L. Gaboury, R. Morisset, Madeleine Ravaoarinoro, L. Sangare |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Capsules Chlamydia trachomatis macromolecular substances medicine.disease_cause Andrology Mice Cations polycyclic compounds medicine Animals Humans Pharmacology (medical) Chlamydiaceae Fallopian Tubes Antibacterial agent Pharmacology Doxycycline Chlamydia biology organic chemicals Lymphogranuloma venereum Uterus technology industry and agriculture Antibody titer Chlamydia Infections biology.organism_classification medicine.disease Antibodies Bacterial Anti-Bacterial Agents carbohydrates (lipids) Infectious Diseases Chlamydiales Liposomes Vagina Immunology Female Genital Diseases Female medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 47:323-331 |
| ISSN: | 1460-2091 |
| DOI: | 10.1093/jac/47.3.323 |
| Popis: | In a previous study, we demonstrated that cationic liposome-encapsulated doxycycline (CaL-Dox) was two-fold more effective than free doxycycline against Chlamydia trachomatis in vitro. Here, we evaluated the effects of two CaL-Dox regimens in comparison with unencapsulated doxycycline on the course of chlamydial genital infection in mice. Progesterone-treated, female CF-1 mice were challenged intravaginally with 1.2 x 10(5) inclusion-forming units (ifu) of C. trachomatis. Two days post-infection, the animals were divided into four treatment groups for im injection of doxycycline at 10 mg/kg body weight bd for 3 (3 Dox) or 7 days (7 Dox), or of CaL-Dox at the same dose level for 3 (3 CaL-Dox) or 7 days (7 CaL-Dox) consecutively. An infected fifth group served as a control and was given an empty CaL preparation. C. trachomatis were isolated after five blind passages from 82% of infected control mice, 61.4% of 3 Dox, 52.2% of 3 CaL-Dox, 29% of 7 Dox and 20% of 7 CaL-Dox animals. Histopathological reactions were found in various tissues of the genital tract in 79.5% of infected control mice, 80.9% of 3 Dox, 65.2% of 3 CaL-Dox, 47.1% of 7 Dox and 25.7% of 7 CaL-Dox animals. Total antichlamydial antibody titres were lower in 7 CaL-Dox mice than in all the other groups (P < 0.005). The results showed that progesterone-treated CF-1 mice are suitable for investigation of both lower and upper genital tract infection with a lymphogranuloma venereum biovar strain of C. trachomatis. Neither 7 CaL-Dox nor 3 CaL-Dox treatment was more effective than unencapsulated 7 Dox doses in the bacteriological cure of chlamydial genital infection in mice. However, 7 CaL-Dox prevented tissue damage in the genital tract significantly more than all the other regimens (P < 0.05). These results suggest that liposome-encapsulated doxycycline, particularly CaL-Dox, may have potential for the clinical treatment of chlamydial infections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|