Controversial use of vitellogenin as a biomarker of endocrine disruption in crustaceans: New adverse pieces of evidence in the copepod Eurytemora affinis

Autor: Céline Boulangé-Lecomte, Stéphanie Olivier, Aurélie Duflot, Nathalie Giusti, Joëlle Forget-Leray, Elena Legrand, Benoit Xuereb, Gauthier Trémolet
Přispěvatelé: Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
DNA
Complementary
Physiology
VTG
Health
Toxicology and Mutagenesis
Endocrine disruption
Zoology
Context (language use)
010501 environmental sciences
Endocrine Disruptors
Toxicology
01 natural sciences
Biochemistry
Copepoda
03 medical and health sciences
Vitellogenin
Vitellogenins
Methyl farnesoate
Ecotoxicology
Animals
14. Life underwater
Amino Acid Sequence
Cloning
Molecular
0105 earth and related environmental sciences
Invertebrate
biology
Base Sequence
Ecology
Estuary
Cell Biology
General Medicine
Biomarker
biology.organism_classification
Crustacean
4-nonylphenol
Crustaceans
Open reading frame
030104 developmental biology
Endocrine disruptor
Gene Expression Regulation
biology.protein
Fatty Acids
Unsaturated
Female
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Copepod
Biomarkers
Water Pollutants
Chemical
Zdroj: Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology
Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, Elsevier, 2017, 201, pp.66-75. ⟨10.1016/j.cbpc.2017.09.011⟩
ISSN: 1532-0456
DOI: 10.1016/j.cbpc.2017.09.011⟩
Popis: International audience; In recent years, the interest in the use of vitellogenin (VTG) as a biomarker of endocrine disruption in fish has led to VTG being considered as a potential tool in invertebrates. Among aquatic invertebrate models in ecotoxicology, the copepods are considered as reference species in marine, estuarine and freshwater ecosystems. In this context, we identified a VTG cDNA in Eurytemora affinis. The Ea-VTG2 cDNA is 5416bp in length with an open reading frame (ORF) of 5310bp that encodes a putative protein of 1769 amino acids residues. Phylogenetic analysis confirmed the hypothesis of a VTG duplication event before the emergence of the copepod species. The analysis of the Ea-VTG2 expression by qPCR in males and females according to their reproductive stages allowed transcript basal levels to be determined. The expression pattern revealed a gradual increase of transcript levels during maturation in females. Important inter-sex differences were observed with a VTG level in males ranging from about 1900- to 6800-fold lower than in females depending on their stage. Moreover, the protein was only detected in ovigerous females. The inducibility of Ea-VTG2 by chemicals was studied in males exposed to either a model of endocrine disruptor in vertebrates i.e. 4-nonylphenol (4-NP) or a crustacean hormone i.e. Methyl Farnesoate (MF), and in males sampled from a multi-contaminated estuary. No induction was highlighted. The VTG should not be considered as an appropriate biomarker in E. affinis as previously suggested for other crustaceans.
Databáze: OpenAIRE
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