Pomegranate (Punica granatum) Juice Shows Antioxidant Activity against Cutaneous Leishmaniasis-Induced Oxidative Stress in Female BALB/c Mice
| Autor: | Manal F. El-Khadragy, Muhammed A Bakhrebah, Ebtesam M. Al-Olayan, Ahmed E. Abdel Moneim, Badriah Alkathiri, Dina M. Metwally |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antioxidant pomegranate (Punica granatum) Health Toxicology and Mutagenesis medicine.medical_treatment Leishmaniasis Cutaneous lcsh:Medicine Pharmacology medicine.disease_cause Article Antioxidants lesions BALB/c Mice cutaneous leishmaniasis 03 medical and health sciences 0302 clinical medicine Cutaneous leishmaniasis Animals treatment Medicine Leishmania major Lythraceae Mice Inbred BALB C biology business.industry lcsh:R Public Health Environmental and Occupational Health Leishmaniasis medicine.disease biology.organism_classification Fruit and Vegetable Juices Oxidative Stress 030104 developmental biology 030220 oncology & carcinogenesis Punica Female Liver function business Oxidative stress |
| Zdroj: | International Journal of Environmental Research and Public Health; Volume 14; Issue 12; Pages: 1592 International Journal of Environmental Research and Public Health, Vol 14, Iss 12, p 1592 (2017) International Journal of Environmental Research and Public Health |
| ISSN: | 1660-4601 |
| Popis: | Leishmania species are parasites that multiply within phagocytes and cause several clinical diseases characterized by single or multiple ulcerations. One of the complications that can induce tissue damage and the resulting scars is caused by secondary bacterial infections. Studies to find new, effective, and safe oral drugs for treating leishmaniasis are being conducted since several decades, owing to the problems associated with the use of antimonials available. Previously, the antiparasitic and antioxidant properties of Punica granatum (pomegranate, P. granatum) have been reported. Therefore, in the present study, we aimed to investigate the antileishmanial activity of pomegranate aqueous juice in vitro and in female BALB/c mice. A 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in Leishmania major promastigotes and alterations in the antioxidant status, liver function, and skin histological changes in L. major-infected mice orally treated with pomegranate juice alone and in combination with the antibiotic ciprofloxacin, were used to investigate the in vitro and in vivo antileishmanial activity of pomegranate juice, respectively. Oral P. granatum juice treatment significantly reduced the average size of cutaneous leishmaniasis lesions compared with that of the untreated mice. This antileishmanial activity of P. granatum was associated with enhanced antioxidant enzyme activities. Histopathological evaluation proved the antileishmanial activity of P. granatum, but did not reveal changes in the treated animals, compared to the positive control. In conclusion, P. granatum shows high and fast antileishmanial activity probably by boosting the endogenous antioxidant activity. |
| Databáze: | OpenAIRE |
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