Passive and Active In Vitro Resorption of Calcium and Magnesium Phosphate Cements by Osteoclastic Cells
Autor: | Andrea Ewald, Jake E. Barralet, Christian Grossardt, Uwe Gbureck, Liam M. Grover |
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Rok vydání: | 2010 |
Předmět: |
Calcium Phosphates
musculoskeletal diseases Blotting Western Biomedical Engineering Magnesium Compounds Osteoclasts chemistry.chemical_element Dentistry Bioengineering Calcium Biochemistry Mass Spectrometry Cell Line Phosphates Bone remodeling Biomaterials Mice X-Ray Diffraction Osteoclast medicine Animals Brushite Calcitonin receptor Tartrate-resistant acid phosphatase Magnesium phosphate business.industry Resorption medicine.anatomical_structure Microscopy Fluorescence chemistry Biophysics business |
Zdroj: | Tissue Engineering Part A. 16:3687-3695 |
ISSN: | 1937-335X 1937-3341 |
Popis: | Biocements are clinically applied materials for bone replacement in non-load-bearing defects. Depending on their final composition, cements can be either resorbed or remain stable at the implantation site. Degradation can occur by two different mechanisms, by simple dissolution (passive) or after osteoclastic bone remodeling (active). This study investigated both the passive and active in vitro resorption behavior of brushite (CaHPO₄ · 2H₂O), monetite (CaHPO₄), calcium-deficient hydroxyapatite (CDHA; Ca₉(PO₄)₅HPO₄OH), and struvite (MgNH₄PO₄ · 6H₂O) cements. Passive resorption was measured by incubating the cement samples in a cell culture medium, whereas active resorption was determined during the surface culture of multinuclear osteoclastic cells derived from RAW 264.7 macrophages. Osteoclast formation was confirmed by showing tartrate resistant acid phosphatase (TRAP) activity on CDHA, brushite, and monetite surfaces, as well as by measuring calcitonin receptor (CT-R) expression as an osteoclast-specific protein by Western blot analysis for struvite ceramics. An absence of passive degradation and only marginally active degradation of0.01% were found for CDHA matrices. For the secondary calcium phosphates brushite and monetite, active degradation was predominant with a cumulative Ca²+ release of 2.02 (1.20) μmol during 13 days, whereas passive degradation released only 0.788 (0.04) μmol calcium ions into the medium. The struvite cement was the most degradable with a passive (active) release of 9.26 (2.92) Mg²+ ions and a total weight loss of 4.7% over 13 days of the study. |
Databáze: | OpenAIRE |
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