Regulation of the strypsin-related proteinase ISP2 by progesterone in endometrial gland epithelium during implantation in mice
| Autor: | Rancourt Sl, Colleen M. O'Sullivan, Rancourt De, Liu Sy |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Embryology
medicine.medical_specialty Ovariectomy Molecular Sequence Data Tryptase Biology Endometrium Polymerase Chain Reaction Epithelium Gene Expression Regulation Enzymologic Mice Endocrinology Hormone Antagonists Proteinase 3 Pregnancy Internal medicine Gene expression medicine Extracellular Animals Blastocyst Amino Acid Sequence Embryo Implantation RNA Messenger Pseudopregnancy Zona pellucida Progesterone Zona Pellucida urogenital system Serine Endopeptidases Obstetrics and Gynecology Cell Biology Sequence Analysis DNA Embryo Mammalian Cell biology Mifepristone medicine.anatomical_structure Reproductive Medicine embryonic structures biology.protein Female |
| Zdroj: | Reproduction (Cambridge, England). 122(2) |
| ISSN: | 1470-1626 |
| Popis: | Hormones prepare the uterus for the arrival and subsequent invasion of the embryo during pregnancy. Extracellular matrix-degrading proteinases and their inhibitors are involved in this integration process. Recent genetic evidence indicates that there is redundancy within the implantation proteinase cascade, indicating that additional proteinases may be involved. Recently, we described a novel implantation serine proteinase (ISP1) gene that encodes the embryo-derived enzyme strypsin, which is necessary for blastocyst hatching in vitro and the initiation of invasion. The evidence presented in the present study indicates that a second proteinase secreted from the uterus also participates in lysis of the zona pellucida. A second implantation serine proteinase gene (ISP2) was isolated, which encodes a related secreted tryptase expressed specifically within uterine endometrial glands. In pseudopregnancy, ISP2 gene expression is dependent on progesterone priming and is inhibited by the antiprogestin RU486. On the basis of similarities between ISP2 gene expression and that of a progesterone-regulated luminal proteinase associated with lysis of the zona pellucida, it is possible that the strypsin-related protein, ISP2, may encode a zona lysin proteinase. |
| Databáze: | OpenAIRE |
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