Synergism Between Keratinocyte Growth Factor and Carboxymethyl Chitosan Reduces Pericardial Adhesions
| Autor: | Sérgio Paulo Campana Filho, Luís Alberto Oliveira Dallan, Noedir Antônio Groppo Stolf, Sérgio Almeida de Oliveira, Paulo Sampaio Gutierrez, Luiz Augusto Ferreira Lisboa, Jackson Brandão Lopes, Luiz Felipe P. Moreira |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine medicine.medical_specialty Fibroblast Growth Factor 7 Heart Diseases Swine medicine.medical_treatment Adhesion (medicine) Tissue Adhesions Andrology chemistry.chemical_compound Cytokeratin medicine Animals Pericardium Sirius Red Saline Chitosan business.industry Growth factor Drug Synergism medicine.disease Surgery medicine.anatomical_structure chemistry Keratinocyte growth factor Cardiology and Cardiovascular Medicine business Mesothelial Cell |
| Zdroj: | The Annals of Thoracic Surgery. 90:566-572 |
| ISSN: | 0003-4975 |
| DOI: | 10.1016/j.athoracsur.2010.03.086 |
| Popis: | Mesothelial injury is the pivot in the development of adhesions. An increase in the proliferation of mesothelial cells was verified by in vitro studies with the use of keratinocyte growth factor (KGF). This study investigated the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions.An induction model of pericardial adhesion was carried out in 24 pigs. Animals were randomly allocated to receive topical application of KGF, KGF + NOCCts, NOCCts, or saline (control). At 8 weeks, intrapericardial adhesions were evaluated and a severity score was established. The time spent to dissect the adhesions and the amount of sharp dissection used, were recorded. Histologic sections were stained with sirius red for a morphometric evaluation using a computer-assisted image analysis system. Cytokeratin AE1/AE3 immunostaining were employed to identify mesothelial cells.The severity score expressed in median (minimum to maximum), in relation to the control group (17 [15 to 18]), was lower in the KGF + NOCCts group (7 [6 to 9], p0.01) followed by the KGF group (11.5 [9 to 12], 0.01p0.05) and the NOCCts group (12 [9 to 14], p0.05). The dissection time was significantly lower in the KGF + NOCCts group (7.1 + or - 0.6 vs 33.9 + or - 9.2 minutes, p0.001). A significantly less sharp dissection was also required in the KGF + NOCCts group. In the adhesion segment, a decreased collagen proportion was found in the KGF + NOCCts group (p0.05). Mesothelial cells were present more extensively in groups in which KGF was delivered (p = 0.01).The use of KGF associated with NOCCts resulted in a synergic action that decreases postoperative pericardial adhesions in a highly significant way. |
| Databáze: | OpenAIRE |
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