Synthesis, photophysical and cellular characterisation of folate and methotrexate labelled luminescent lanthanide complexes
| Autor: | Sally E. Plush, Doug A. Brooks, Zhangli Du, Christie A. Bader, Xun Li, Minqi Li, Jing Sun |
|---|---|
| Přispěvatelé: | Du, Zhangli, Sun, Jing, Bader, Christie A, Brooks, Doug A, Li, Minqi, Li, Xun, Plush, Sally E |
| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
Lanthanide emissive europium(III) Luminescence characterisation of folate Cell Survival Stereochemistry chemistry.chemical_element 010402 general chemistry Lanthanoid Series Elements 01 natural sciences Biochemistry Inorganic Chemistry Folic Acid Coordination Complexes Cell Line Tumor Humans heterocyclic compounds Chelation skin and connective tissue diseases Cytotoxicity Receptor Cell Proliferation Molecular Structure Cytotoxins 010405 organic chemistry terbium(III) complexes 0104 chemical sciences Methotrexate chemistry Folate receptor Cancer cell Europium HeLa Cells Conjugate |
| Zdroj: | Journal of Inorganic Biochemistry. 178:32-42 |
| ISSN: | 0162-0134 |
| Popis: | In this work we have developed a series of highly emissive europium(III) and terbium(III) complexes tethered to either folic acid (FA) or methotrexate (MTX), with the aim of developing visual probes that enable the imaging of folate receptors in cancer cells. The synthesis, photophysical properties and cellular behaviour are reported for four new lanthanide Ln(III) complexes, where either FA or MTX are tethered to 1,4,7-tris(carbonylmethyl)-10-(4′-quinolineacetic acid, (7′-acetamido)-1′,2′-dihydro-2′-oxo)-1,4,7,10-tetraazacyclododecane Ln(III) complex, and Ln(III) = Eu(III) or Tb(III); herein referred to as Eu-FA, Eu-MTX, Tb-FA or Tb-MTX. All four complexes were found to be sensitive to the presence of the folate receptor in a range of cell lines. The MTX conjugates showed different cellular specificity in an oral adenosquamous carcinoma cell line (CAL-27) compared with the analogous FA conjugates. This suggests that it is viable to explore differences in folate receptors using folate vs. anti-folate probes, with labels that have different emissive properties (e.g. Eu-FA vs. Tb-MTX). The MTX complexes were found to be the most cytotoxic, with Eu-MTX showing greater cytotoxicity than free MTX or the isostructural Tb-MTX. This suggested that there could be a synergistic effect on toxicity for the Eu(III) chelate and the MTX components of the complex. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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