Quantitative analysis of S3341 in human plasma and urine by combined gas chromatography—negative ion chemical ionization mass spectrometry: 15 month inter-day precision and accuracy validation
| Autor: | H. L. Ung, J. Girault, J. B. Fourtillan, A. Mignot, M. A. Lefebvre |
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| Rok vydání: | 1987 |
| Předmět: |
Quality Control
Detection limit Accuracy and precision Chemical ionization Chromatography Chemistry Analytical chemistry Rilmenidine Gas Chromatography-Mass Spectrometry Ion Pharmacokinetics Humans Selected ion monitoring Gas chromatography Adrenergic alpha-Agonists Oxazoles Quantitative analysis (chemistry) Spectroscopy |
| Zdroj: | Biological Mass Spectrometry. 14:289-293 |
| ISSN: | 1096-9888 0887-6134 |
| Popis: | A new quantitative assay for the determination of S3341, an alpha-2 agonist antihypertensive drug, has been developed using combined gas chromatography-negative ion chemical ionization mass spectrometry. The [M]-. ions from TFA derivatives of S3341 (m/z 276) and the internal standard (2H4)S3341 (m/z 280) are monitored simultaneously by selected ion monitoring. For S3341 concentrations ranging from the limit of detection (0.2 ng ml-1 using 1 ml of plasma) to 5 ng ml-1, the average assay precision (CV) is approximately 7% while the average assay accuracy (percentage of error) is 4%. Validation of the day-to-day precision and accuracy was realized after analysing control plasma samples (n = 295) concurrently with the biological samples collected during the pharmacokinetic studies conducted over 15 months. The average day-to-day precision (CV) and accuracy (percentage of error) are 10% and 6% respectively, thus indicating that this assay procedure routinely provides reliable analytical data. |
| Databáze: | OpenAIRE |
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