Gamma-secretase subunits associate in intracellular membrane compartments in Arabidopsis thaliana
| Autor: | Krzysztof Leśniewicz, Michał Michalak, Tomasz Skrzypczak, Przemysław Wojtaszek, J. Ross Walker, Michalina Smolarkiewicz, Gwyneth C. Ingram |
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| Přispěvatelé: | Smolarkiewicz, Michalina, Wojtaszek, Przemyslaw, Adam Mickiewicz University in Poznań (UAM), Institute of Molecular Plant Sciences, University of Edinburgh, Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Ministry of Science and Higher Education [NN303 360735, NN303 813540], National Science Centre [2012/05/B/NZ3/01026], LeverhulmeTrust, École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
senescence
Authophagy complex assembly endomembrane system gamma-secretase intramembrane protease localization presenilin vesicular trafficking Physiology Intramembrane protease secretase [SDV]Life Sciences [q-bio] Molecular Sequence Data Arabidopsis sénescence Plant Science autophagie Presenilin 03 medical and health sciences Amyloid precursor protein [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Endomembrane system Amino Acid Sequence Phylogeny Gamma secretase 030304 developmental biology 0303 health sciences biology Arabidopsis Proteins Protoplasts 030302 biochemistry & molecular biology fungi protéase Intracellular Membranes biology.organism_classification Molecular biology Cell biology Membrane protein système endomembranaire biology.protein Amyloid Precursor Protein Secretases Sequence Alignment Amyloid precursor protein secretase Research Paper |
| Zdroj: | Journal of Experimental Botany 12 (65), 3015-3027. (2014) Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2014, 65 (12), pp.3015-3027. ⟨10.1093/jxb/eru147⟩ Journal of Experimental Botany, 2014, 65 (12), pp.3015-3027. ⟨10.1093/jxb/eru147⟩ |
| ISSN: | 0022-0957 1460-2431 |
| DOI: | 10.1093/jxb/eru147⟩ |
| Popis: | Summary In animals, gamma-secretase is crucial for regulation of important developmental processes. Here, we show that genes encoding components of this complex are present in Arabidopsis, and the complex undergoes complicated assembly in the endomembrane system. This complex may have some role in autophagy. Gamma-secretase is a multisubunit complex with intramembrane proteolytic activity. In humans it was identified in genetic screens of patients suffering from familial forms of Alzheimer’s disease, and since then it was shown to mediate cleavage of more than 80 substrates, including amyloid precursor protein or Notch receptor. Moreover, in animals, γ-secretase was shown to be involved in regulation of a wide range of cellular events, including cell signalling, regulation of endocytosis of membrane proteins, their trafficking, and degradation. Here we show that genes coding for γ-secretase homologues are present in plant genomes. Also, amino acid motifs crucial for γ-secretase activity are conserved in plants. Moreover, all γ-secretase subunits: PS1/PS2, APH-1, PEN-2, and NCT colocalize and interact with each other in Arabidopsis thaliana protoplasts. The intracellular localization of γ-secretase subunits in Arabidopsis protoplasts revealed a distribution in endomembrane system compartments that is consistent with data from animal studies. Together, our data may be considered as a starting point for analysis of γ-secretase in plants. |
| Databáze: | OpenAIRE |
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