Using targeted next-generation sequencing to characterize genetic differences associated with insecticide resistance in Culex quinquefasciatus populations from the southern U.S

Autor: John Phan, Enas Ghallab, Harry M. Savage, Linda Kothera, Rebecca J. Clark, Mark J. Delorey
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Insecticides
Hydrolases
Disease Vectors
Mosquitoes
Biochemistry
Insecticide Resistance
0302 clinical medicine
Medicine and Health Sciences
Copy-number variation
Genetics
education.field_of_study
Multidisciplinary
biology
Organic Compounds
Esterases
Arizona
Eukaryota
High-Throughput Nucleotide Sequencing
Agriculture
Texas
Organophosphates
Enzymes
Insects
Chemistry
Culex
Infectious Diseases
Physical Sciences
Malathion
Medicine
Agrochemicals
SNP array
Research Article
Ethers
Arthropoda
Science
030231 tropical medicine
Population
Single-nucleotide polymorphism
Culex Quinquefasciatus
Polymorphism
Single Nucleotide
DNA sequencing
Molecular Genetics
03 medical and health sciences
parasitic diseases
Animals
education
Molecular Biology
Permethrin
Organic Chemistry
fungi
Organisms
Chemical Compounds
Gene Amplification
Biology and Life Sciences
Proteins
Ion semiconductor sequencing
biology.organism_classification
Louisiana
Invertebrates
Culex quinquefasciatus
Insect Vectors
Species Interactions
030104 developmental biology
Mutation
Enzymology
Zdroj: PLoS ONE, Vol 14, Iss 7, p e0218397 (2019)
PLoS ONE
ISSN: 1932-6203
Popis: Resistance to insecticides can hamper the control of mosquitoes such as Culex quinquefasciatus, known to vector arboviruses such as West Nile virus and others. The strong selective pressure exerted on a mosquito population by the use of insecticides can result in heritable genetic changes associated with resistance. We sought to characterize genetic differences between insecticide resistant and susceptible Culex quinquefasciatus mosquitoes using targeted DNA sequencing. To that end, we developed a panel of 122 genes known or hypothesized to be involved in insecticide resistance, and used an Ion Torrent PGM sequencer to sequence 125 unrelated individuals from seven populations in the southern U.S. whose resistance phenotypes to permethrin and malathion were known from previous CDC bottle bioassay testing. Data analysis consisted of discovering SNPs (Single Nucleotide Polymorphism) and genes with evidence of copy number variants (CNVs) statistically associated with resistance. Ten of the seventeen genes found to be present in higher copy numbers were experimentally validated with real-time PCR. Of those, six, including the gene with the knock-down resistance (kdr) mutation, showed evidence of a ≥ 1.5 fold increase compared to control DNA. The SNP analysis revealed 228 unique SNPs that had significant p-values for both a Fisher's Exact Test and the Cochran-Armitage Test for Trend. We calculated the population frequency for each of the 64 nonsynonymous SNPs in this group. Several genes not previously well characterized represent potential candidates for diagnostic assays when further validation is conducted.
Databáze: OpenAIRE
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