Cloning and sequencing of a K+ transport gene (trk A) from the marine bacterium Vibrio alginolyticus
| Autor: | Yasuhiro Matsuba, Ian R. Booth, Naoya Yamamuro, Tatsunosuke Nakamura, Tsutomu Unemoto |
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| Rok vydání: | 1994 |
| Předmět: |
Potassium ion transport
Molecular Sequence Data Biophysics Marine Biology Biology medicine.disease_cause Biochemistry Bacterial Proteins Structural Biology Escherichia coli Genetics medicine Amino Acid Sequence Cloning Molecular Receptor trkA Peptide sequence Gene Vibrio Vibrio alginolyticus Base Sequence Genetic Complementation Test Nucleic acid sequence Membrane Proteins Biological Transport Sequence Analysis DNA biology.organism_classification Molecular biology Open reading frame nervous system Genes Bacterial Potassium Carrier Proteins Water Microbiology |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1219:701-705 |
| ISSN: | 0167-4781 |
| Popis: | A gene has been cloned from the marine bacterium Vibrio alginolyticus that functionally complements a mutant strain of Escherichia coli , TK420, defective in K + transport genes ( kdpABC, trkD, trkA ). The cloned Vibrio gene allowed TK420 to grow in a synthetic medium containing less than 10 mM K + and concomitantly led to an increase in K + uptake activity. The nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 458 amino acid sequence and molecular mass of 50 122 Da. This gene has 71% homology to trkA gene at the DNA level from E. coli and the deduced amino acid sequence is 79% identical with E. coli TrkA, implying that V. alginolyticus has a trkA -like gene as a component of K + transport systems. |
| Databáze: | OpenAIRE |
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