Occurrence of members of the insulin superfamily in central nervous system and digestive tract of protochordates
| Autor: | Vicki R. Sara, Sture Falkmer, Manfred Reinecke, Katarina Drakenberg, D. Betzler |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Central Nervous System
Branchiostoma medicine.medical_specialty medicine.medical_treatment Peptide Biology Radioligand Assay Chordata Nonvertebrate Internal medicine medicine Animals Insulin Ciona intestinalis Insulin-Like Growth Factor I chemistry.chemical_classification Chromatography Branchiostoma lanceolatum Radioimmunoassay General Medicine biology.organism_classification Immunohistochemistry Molecular biology Ciona medicine.anatomical_structure Endocrinology chemistry Rabbits Anatomy General Agricultural and Biological Sciences Pancreas Digestive System |
| Zdroj: | Histochemistry. 99:277-285 |
| ISSN: | 0301-5564 |
| DOI: | 10.1007/bf00269100 |
| Popis: | Antisera specific for mammalian insulin-like growth factor 1 (IGF-1) and mammalian insulin and the double immunofluorescence technique were used for this study. IGF-1-like-immunoreactivity was localized in entero-endocrine cells in the gastro-intestinal tract of the protochordates Ciona intestinalis and Branchiostoma lanceolatum. Some of the specimens also showed IGF-1-like-immunoreactive (-IR) perikarya and fibers in the central nervous system. Whilst in rat endocrine pancreas, IGF-1-IR and insulin-IR occurred in different cell populations, in Ciona and Branchiostoma the vast majority of entero-endocrine cells and central neurons were IGF-1-like- +insulin-IR. A minor portion exhibited IGF-1-like-IR alone. For further characterization of the IGF-1-like-IR material, in Ciona intestinalis, peptides related to IGF-1 were identified by radioimmunoassay and gel chromatography. In accordance with the immunohistochemical results, IGF-I-like-IR was detected both in cerebral ganglion and in gastro-intestinal tract. Using acid gel chromatography, in Ciona gastro-intestinal tract the IGF-1-like-IR was found to occur in two peaks, with apparent molecular weights of approximately 16 kDa and 3 kDa. Absorption studies with insulin- and IGF-related peptides, with crude extracts and the peak material obtained after gel chromatography, indicated that the IGF-1-like peptides in Ciona are different from mammalian insulin and IGF-1. The findings are in accordance with the presence of a common insulin/IGF precursor molecule in protochordates. |
| Databáze: | OpenAIRE |
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