Diagnostic Efficiency of Serum and Urine Procathepsin B and Cathepsin B in Patients with Carcinoma of the Urinary Bladder
| Autor: | Karel Kotaska, Stepan Vesely, Marek Babjuk, Richard Prusa, Pavel Dusek |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male medicine.medical_specialty Procathepsin B Urology Urine General Biochemistry Genetics and Molecular Biology Cathepsin B chemistry.chemical_compound Carcinoma Biomarkers Tumor Medicine Humans In patient Aged Aged 80 and over Creatinine Carcinoma Transitional Cell Enzyme Precursors Urinary bladder business.industry Diagnostic marker Middle Aged medicine.disease medicine.anatomical_structure chemistry Urinary Bladder Neoplasms Area Under Curve Female business |
| Zdroj: | Clinical laboratory. 62(9) |
| ISSN: | 1433-6510 |
| Popis: | BACKGROUND The aim of the study was to evaluate the diagnostic efficiency of cathepsins B (cathepsin B and procathepsin B) in patients with transient cell carcinoma of the urinary bladder. METHODS Serum and urine concentrations of cathepsin B and procathepsin B were measured by two commercially available enzymatic immunoassays in a group of 125 patients with bladder cell carcinoma without metastases and in a group of 72 healthy individuals. Concentrations in urine were adjusted to creatinine. RESULTS Concentrations of both cathepsin B and procathepsin B in serum and urine were significantly elevated in patients with bladder cell carcinoma (p < 0.0001 for U-procathepsin B, U-procathepsin B/creatinine, and U-cathepsin B/creatinine, p = 0.0001 for U-cathepsin B, p = 0.0002 for S-procathepsin B and p = 0.02 for S-cathepsin B). Comparison of all diagnostic efficiencies of cathepsin B and procathepsin B in serum and in urine showed the best diagnostic accuracy for procathepsin B in urine (AUC = 0.81 vs. 0.50). The ratio of U-procathepsin B/creatinine was also more efficient than the ratio of U-cathepsin B/creatinine (AUC = 0.81 vs. AUC = 0.70). The diagnostic efficiencies of both parameters in serum were low (S-procathepsin B: AUC = 0.50, S-cathepsin B: AUC = 0.60). U-procathepsin B and U-procathepsin B/creatinine ratio show significantly better diagnostic efficiency in patients with invasive bladder tumors than other parameters (S-procathepsin B, S-cathepsin B, U-cathepsin B and U-Cathepsin B/creatinine; U-procathepsin B: AUC = 0.82, U-procathepsin B/creatinine: AUC = 0.86, S-procathepsin B and cathepsin B: AUC = 0.51 - 0.68). CONCLUSIONS Procathepsin B concentration in urine is a valuable diagnostic marker in patients with bladder cell carcinoma. |
| Databáze: | OpenAIRE |
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