Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells
| Autor: | Tonni Agustiono Kurniawan, Nursyamirah Abd Razak, Seema Zareen, Alan Han Kiat Ong, Muhammad Nadeem Akhtar, Zhi Xiong Chong, Swee Keong Yeap, Norlaily Mohd Ali, Lily Boo, Noorjahan Banu Alitheen, Stephanie Y. L. Ng, Ram Avtar, Wan Yong Ho |
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| Rok vydání: | 2021 |
| Předmět: |
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6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Pharmaceutical Science Estrogen receptor Analytical Chemistry lcsh:QD241-441 03 medical and health sciences breast cancer 0302 clinical medicine lcsh:Organic chemistry Drug Discovery microRNA Cytotoxic T cell Viability assay Physical and Theoretical Chemistry skin and connective tissue diseases miRNA 030304 developmental biology 0303 health sciences Chemistry Organic Chemistry apoptosis Cell cycle Real-time polymerase chain reaction MCF-7 Chemistry (miscellaneous) Apoptosis 030220 oncology & carcinogenesis Cancer research Molecular Medicine |
| Zdroj: | Molecules, Vol 26, Iss 1277, p 1277 (2021) Molecules Volume 26 Issue 5 |
| ISSN: | 1420-3049 |
| Popis: | (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes. |
| Databáze: | OpenAIRE |
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