Serum anti-p53 in relation to mutations across the entire translated p53 gene in colorectal carcinomas
| Autor: | Mats Inganäs, Ann Forslund, Ulf Kressner, Kent Lundholm, Gudrun Lindmark |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Cancer Research Tumor suppressor gene Antibodies Neoplasm DNA Mutational Analysis Enzyme-Linked Immunosorbent Assay Biology Gene mutation medicine.disease_cause Immunoenzyme Techniques Exon Gene expression medicine Humans Missense mutation Gene Aged Neoplasm Staging Aged 80 and over Mutation Middle Aged Flow Cytometry Genes p53 Neoplasm Proteins Oncology Cancer research biology.protein Female Tumor Suppressor Protein p53 Antibody Colorectal Neoplasms |
| Zdroj: | International Journal of Oncology. |
| ISSN: | 1791-2423 1019-6439 |
| Popis: | Previous reports have claimed that antibodies to mutated p53 protein indicate poor outcome in malignant disease. The mechanism behind this highly specific process is unclear, although it has been claimed that certain DNA alterations are prone to induction of immune response, since wild-type p53 is almost never immunogenic. The aim of the present analysis was to evaluate whether the presence of anti-p53 was statistically significantly related to any certain DNA alterations in the entirely expressed p53 gene in primary tumors of colorectal cancer. P53 serum antibodies were determined by an enzyme linked immunosorbant assay (ELISA). P53 antibodies were detected in serum of 24 of 88 patients (27%). Twenty-two of 24 (92%) sero-positive patients had mutations in their p53 gene while only 22 of 64 (34%) sero-negative patients had p53 mutations (p |
| Databáze: | OpenAIRE |
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