Human herpesvirus 6A U27 plays an essential role for the virus propagation
| Autor: | Aika Wakata, Yasuko Mori, Lidya Handayani Tjan, Anna Lystia Poetranto, Mitsuhiro Nishimura, Jun Arii |
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| Rok vydání: | 2020 |
| Předmět: |
Human cytomegalovirus
DNA Replication Fever DNA polymerase viruses Herpesvirus 6 Human Immunology Mutant Genome Viral Virus Replication Microbiology Cell Line 03 medical and health sciences Viral Proteins Virology medicine Exanthema Subitum Humans Frameshift Mutation Gene 030304 developmental biology 0303 health sciences biology DNA synthesis 030306 microbiology DNA replication virus diseases Processivity biochemical phenomena metabolism and nutrition medicine.disease HEK293 Cells Viral replication DNA Viral biology.protein |
| Zdroj: | Microbiology and immunologyREFERENCES. 64(10) |
| ISSN: | 1348-0421 |
| Popis: | Human herpesvirus 6A (HHV-6A) is a member of the genus Roseolovirus and the subfamily Betaherpesvirinae. It is similar to and human cytomegalovirus (HCMV). HHV-6A encodes a 41 kDa nuclear phosphoprotein, U27, which acts as a processivity factor in the replication of the viral DNA. HHV-6A U27 has 43% amino acid sequence homology with HCMV UL44, which is important for DNA replication. A previous study on HHV-6A U27 revealed that it greatly increases the in vitro DNA synthesis activity of HHV-6A DNA polymerase. However, the role of U27 during the HHV-6A virus replication process remains unclear. In this study, we constructed a U27-deficient HHV-6A mutant (HHV-6ABACU27mut) with a frameshift insertion at the U27 gene using an HHV-6A bacterial artificial chromosome (BAC) system. Viral reconstitution from the mutant BAC DNA was not detected, in contrast to the wild type and the revertant from the U27 mutant. This suggests that U27 plays a critical role in the life cycle of HHV-6A. |
| Databáze: | OpenAIRE |
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