Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography
| Autor: | Michelle A. Digman, Albert F. Yee, Elena I. Liang, Emma J. Mah |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
physiology [Cell Adhesion] Surface Properties Statistics as Topic Biophysics Focal adhesion assembly 02 engineering and technology Mechanotransduction Cellular Biochemistry Article Focal adhesion 03 medical and health sciences Cell Movement Cell Adhesion Medicine and Health Sciences Nanotopography Cell adhesion Paxillin Nanopillar Focal Adhesions chemistry ultrastructure [Nanoparticles] biology Chemistry physiology [Focal Adhesions] Adhesion 021001 nanoscience & nanotechnology Cell biology 030104 developmental biology physiology [Mechanotransduction Cellular] biology.protein Nanoparticles physiology [Cell Movement] 0210 nano-technology Mesenchymal cell migration |
| Zdroj: | Liang, Elena I; Mah, Emma J; Yee, Albert F; & Digman, Michelle A. (2017). Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography.. Integrative biology : quantitative biosciences from nano to macro, 9(2), 145-155. UC Irvine: Institute for Clinical and Translational Science. Retrieved from: http://www.escholarship.org/uc/item/77w3t1p6 |
| ISSN: | 1757-9708 1757-9694 |
| Popis: | Selective cell adhesion is desirable to control cell growth and migration on biomedical implants. Mesenchymal cell migration is regulated through focal adhesions (FAs) and can be modulated by their microenvironment, including changes in surface topography. We use the Number and Molecular Brightness (N&B) imaging analysis to provide a unique perspective on FA assembly and disassembly. This imaging analysis generates a map of real-time fluctuations of protein monomers, dimers, and higher order aggregates of FA proteins, such as paxillin during assembly and disassembly. We show a dynamic view of how nanostructured surfaces (nanoline gratings or nanopillars) regulate single molecular dynamics. In particular, we report that the smallest nanopillars (100 nm spacing) gave rise to a low percentage population of disassembly adhesion cluster size of ~2 paxillin proteins/cluster whereas the larger nanopillars (380 nm spacing) gave rise to a much larger population of larger disassembling cluster of ~3–5 paxillin proteins. Cells were more motile on the smaller nanopillars (spaced 100–130 nm apart) compared to all other surfaces studied. Thus, physical nanotopography influences cell motility, adhesion size, and adhesion assembly and disassembly. We report for the first time, with single molecular detection, how nanotopography influences cell motility and protein reorganization in adhesions. |
| Databáze: | OpenAIRE |
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