De novo design of symmetric ferredoxins that shuttle electrons in vivo
| Autor: | Vikas Nanda, Alexei M. Tyryshkin, George N. Bennett, Paul G. Falkowski, Andrew C. Mutter, Jonathan J. Silberg, Saroj Poudel, Ian J. Campbell |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
media_common.quotation_subject Electron 010402 general chemistry medicine.disease_cause 01 natural sciences Asymmetry Electron Transport Evolution Molecular 03 medical and health sciences Electron transfer In vivo Gene Duplication Consensus Sequence Escherichia coli medicine Molecule Phylogeny Ferredoxin media_common Physics Multidisciplinary Escherichia coli Proteins Biological Sciences 0104 chemical sciences 030104 developmental biology Metabolic Engineering Biophysics Ferredoxins Sequence space (evolution) Metabolic Networks and Pathways |
| Zdroj: | Proceedings of the National Academy of Sciences. 116:14557-14562 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1905643116 |
| Popis: | Significance Early life is thought to have evolved from simple building blocks that were propagated through gene duplication events. A classic example is the small soluble iron-sulfur containing protein, bacterial ferredoxin, which is an asymmetric dimer, an essential component of many extant electron transfer chains and has ancient origins. To probe the theoretical gene duplication origins of bacterial ferredoxins, we designed a series of synthetic symmetric constructs. All designs bound two iron-sulfur clusters and were able to support electron transfer between a pair of oxidoreductases in vivo in Escherichia coli . Our results strongly suggest that simple, symmetric ancestral proteins probably evolved early in Earth’s history and can be engineered to facilitate functional electron transfer in synthetic metabolic pathways. |
| Databáze: | OpenAIRE |
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