Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes
| Autor: | Archana Gengatharan, Armen Saghatelyan, Alina Marymonchyk, Sarah Malvaut |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Science (General)
Biology NSC division General Biochemistry Genetics and Molecular Biology neuroscience Mice 03 medical and health sciences Q1-390 0302 clinical medicine Neural Stem Cells In vivo Live cell imaging stem cells Protocol Animals reproductive and urinary physiology 030304 developmental biology Endoscopes 0303 health sciences General Immunology and Microbiology behavior General Neuroscience Neural stem cell 3. Good health nervous system diseases nervous system microscopy Calcium Stem cell biological phenomena cell phenomena and immunity Neuroscience 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | STAR Protocols, Vol 2, Iss 2, Pp 100596-(2021) STAR Protocols |
| ISSN: | 2666-1667 |
| Popis: | Summary During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca2+ activity can be performed continuously for 2–3 days and even up to several months. For complete details on the use and execution of this protocol, please refer to Gengatharan et al. (2021). Graphical abstract Highlights • A protocol for mini-endoscopic imaging of adult neural stem cells (NSCs) • Live imaging of NSCs activation in freely behaving mice • In vivo mini-endoscopic imaging and analysis of Ca2+ dynamics in NSCs • A protocol for labeling adult NSCs based on coincident activity of two promoters During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca2+ activity can be performed continuously for 2–3 days and even up to several months. |
| Databáze: | OpenAIRE |
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