RNA Helicase Important for Listeria monocytogenes Hemolytic Activity and Virulence Factor Expression
| Autor: | Jörgen Johansson, Sakura Netterling, Caroline Bäreclev, Karolis Vaitkevicius |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Virulence Factors Immunology Bacterial Toxins Virulence Biology medicine.disease_cause Microbiology Hemolysis Virulence factor Cell Line 03 medical and health sciences Hemolysin Proteins Mice Listeria monocytogenes Bacterial Proteins medicine Animals Humans RRNA processing Heat-Shock Proteins Listeriolysin O RNA Membrane Proteins Gene Expression Regulation Bacterial Ribosomal RNA RNA Helicase A Molecular Pathogenesis 030104 developmental biology Infectious Diseases Parasitology Caco-2 Cells Protein Processing Post-Translational RNA Helicases Peptide Termination Factors |
| Popis: | RNA helicases have been shown to be important for the function of RNA molecules at several levels, although their putative involvement in microbial pathogenesis has remained elusive. We have previously shown that Listeria monocytogenes DExD-box RNA helicases are important for bacterial growth, motility, ribosomal maturation, and rRNA processing. We assessed the importance of the RNA helicase Lmo0866 (here named CshA) for expression of virulence traits. We observed a reduction in hemolytic activity in a strain lacking CshA compared to the wild type. This phenomenon was less evident in strains lacking other RNA helicases. The reduced hemolysis was accompanied by lower expression of major listerial virulence factors in the Δ cshA strain, mainly listeriolysin O, but also to some degree the actin polymerizing factor ActA. Reduced expression of these virulence factors in the strain lacking CshA did not, however, correlate with a decreased level of the virulence regulator PrfA. When combining the Δ cshA knockout with a mutation creating a constitutively active PrfA protein (PrfA*), the effect of the Δ cshA knockout on LLO expression was negated. These data suggest a role for the RNA helicase CshA in posttranslational activation of PrfA. Surprisingly, although the expression of several virulence factors was reduced, the Δ cshA strain did not demonstrate any reduced ability to infect nonphagocytic cells compared to the wild-type strain. |
| Databáze: | OpenAIRE |
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