Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA
| Autor: | Zaide Zhou, Hongyan Yuan, Chunhua Liu, Dan Xiao, Xiupei Yang |
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| Rok vydání: | 2007 |
| Předmět: |
Diffraction
Materials science Analytical chemistry Nanoparticle Nanotechnology lcsh:Chemical technology Biochemistry Full Research Paper Silver nanoparticle Analytical Chemistry chemistry.chemical_compound silver lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Detection limit Range (particle radiation) nanoparticle Fluorescence Atomic and Molecular Physics and Optics Silver nitrate chemistry calf thymus DNA (ct-DNA) Transmission electron microscopy fluorescence |
| Zdroj: | Sensors, Vol 7, Iss 5, Pp 708-718 (2007) Sensors (Basel, Switzerland) Sensors; Volume 7; Issue 5; Pages: 708-718 |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s7050708 |
| Popis: | new method to prepare silver nanoparticles was reported in this paper, which based on aqueous-gaseous phase reaction of silver nitrate solution and ammonia gas. The proposed method is effective, rapid, and convenient. Transmission electron microscope (TEM) and X-ray diffraction (XRD) were used to characterize the structure of the particles, respectively. It was found that the average diameter of the particles was about 10 nm and the shape was spherical. And the strong fluorescence signal of the silver nanoparticles solution can be quenched after the adding of the calf thymus DNA (ct-DNA) solution. The quenched fluorescence intensity was linear with the concentration of ct-DNA in the range of 0.5 to 5.0 × 104 ng mL-1 with a detection limit of 0.3 ng mL-1. |
| Databáze: | OpenAIRE |
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