Diagnosis of acute typhus infection using the polymerase chain reaction
| Autor: | S. F. Paparello, C. W. Tibbs, Mitchell Carl, Gregory A. Dasch, Michael E. Dobson |
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| Rok vydání: | 1990 |
| Předmět: |
Adult
DNA Bacterial Male Epidemic typhus animal structures Rocky Mountain spotted fever Molecular Sequence Data Rickettsia rickettsii Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology law.invention History and Philosophy of Science law medicine Immunology and Allergy Humans Rickettsia prowazekii Polymerase chain reaction Base Sequence biology General Neuroscience Hybridization probe bacterial infections and mycoses medicine.disease biology.organism_classification Virology Infectious Diseases Rickettsiosis Acute Disease bacteria Primer (molecular biology) DNA Probes Typhus Typhus Epidemic Louse-Borne |
| Zdroj: | Annals of the New York Academy of Sciences. 590 |
| ISSN: | 0077-8923 |
| Popis: | The first use of the polymerase chain reaction (PCR) for the diagnosis of an acute rickettsial infection is described. A primer pair derived from the 17-kDa antigen sequence of Rickettsia rickettsii gave specific amplification of a 434-base pair DNA fragment from the genome of Rocky Mountain spotted fever and endemic and epidemic typhus. The assay could detect as few as 30 rickettsiae. Detection of PCR-amplified DNA with a nonradioactive DNA probe confirmed an acute infection with Rickettsia prowazekii. |
| Databáze: | OpenAIRE |
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