Rapid Identification of Mycobacterium tuberculosis Beijing Genotypes on the Basis of the Mycobacterial Interspersed Repetitive Unit Locus 26 Signature
Autor: | Sriramula Srinivas, Leonardo Antonio Sechi, Seyed E. Hasnain, Niyaz Ahmed, K. Rajender Rao |
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Rok vydání: | 2006 |
Předmět: |
DNA
Bacterial Microbiology (medical) Tuberculosis Genotype biology Outbreak Mycobacteriology and Aerobic Actinomycetes Locus (genetics) Mycobacterium tuberculosis Interspersed Repetitive Sequences biology.organism_classification medicine.disease Virology Bacterial Typing Techniques Microbiology Beijing medicine Humans Letters to the Editor Genotyping |
Zdroj: | Journal of Clinical Microbiology. 44:274-277 |
ISSN: | 1098-660X 0095-1137 |
DOI: | 10.1128/jcm.44.1.274-277.2006 |
Popis: | Mycobacterium tuberculosis Beijing strains are prevalent in many parts of the world and often give rise to large institutional outbreaks. Such highly transmissible strains, often associated with multidrug resistance, are likely underrepresented in outbreaks reported from developing countries, mainly due to nonavailability of fast detection methods suitable in epidemiological surveillance studies. We evaluated a PCR assay based on amplification of mycobacterial interspersed repetitive unit locus 26 as a stand-alone method for unambiguous identification of Beijing strains. The method was used on blinded samples from 10 standard strains whose Beijing status was already confirmed by spoligotyping. All 10 strains were accurately identified, and their profiles were corroborated successfully with spoligotypes. The method was also applied to 70 different non-Beijing clinical isolates from different countries to allow discrimination of isolates. Owing to its accuracy, simplicity, and rapidity, the assay can be employed in laboratory-level testing of isolates linked to certain outbreaks. The test can also be adopted for direct application on clinical samples to save time on culturing bacilli for genotyping. |
Databáze: | OpenAIRE |
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