The acridine orange fluorochrome microassay: a new technique for quantitation of neutrophil function in burned patients
| Autor: | Joseph V. Boykin, B. W. Haynes, Carpenter Ab, Stephen L. Crute |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Adult
Time Factors business.industry Neutrophils Phagocytosis Acridine orange Bacterial killing Clinical course Middle Aged Critical Care and Intensive Care Medicine Acridine Orange Microbiology chemistry.chemical_compound chemistry Phagocyte Bactericidal Dysfunction Medicine Humans Surgery business Burns |
| Zdroj: | The Journal of trauma. 26(4) |
| ISSN: | 0022-5282 |
| Popis: | A new assay for determination of neutrophil bacterial killing and phagocytosis is presented. The acridine orange (AO) fluorochrome microassay is a simple, reliable technique for assessing polymorphonuclear (PMN) function. It requires small amounts of blood and provides a rapid and reproducible quantitation of neutrophil activity. Using this technique, bacterial killing and phagocytosis were assessed in a group of five severely burned patients admitted to the Medical College of Virginia Burn Unit. All patients studied demonstrated a significant decrease in bacterial killing at some point during their clinical course. The AO assay was found to be a reliable and effective means of quantitating PMN bacterial phagocytosis and killing in this group of burned patients. |
| Databáze: | OpenAIRE |
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