Cell and extracellular matrix interaction models in benign mesothelial and malignant pleural mesothelioma cells in 2D and 3D in-vitro
| Autor: | Stefanos Giannopoulos, Eleftherios Papazoglou, Lydia Giannakou, Eleanna Pitaraki, Konstantinos I. Gourgoulianis, Sotirios G. Zarogiannis, Olympia Kouliou, Chrissi Hatzoglou, Erasmia Rouka, Rajesh Jagirdar, Sotirios I. Sinis |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell type Epithelial-Mesenchymal Transition Lung Neoplasms Physiology Cell Extracellular matrix 03 medical and health sciences 0302 clinical medicine Physiology (medical) Cell Line Tumor medicine Biomarkers Tumor Humans Cell adhesion Pharmacology biology Chemistry Mesothelioma Malignant Pleural cavity Extracellular Matrix Fibronectin 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Cancer research biology.protein Mesothelial Cell |
| Zdroj: | Clinical and experimental pharmacologyphysiologyREFERENCES. 48(4) |
| ISSN: | 1440-1681 |
| Popis: | Malignant pleural mesothelioma (MPM) is an aggressive tumour that grows in the pleural cavity. MPM spheroids released in the pleural fluid can form new tumour foci. Cell-cell, cell-extracellular matrix (ECM) interactions in 2D and 3D impact malignant cell behaviour during cell adhesion, migration, proliferation and epithelial-mesenchymal transition (EMT). In this study, epithelioid, biphasic and sarcomatoid MPM cell types as well as benign mesothelial cells were tested with regards to the above phenotypes. Fibronectin (FN) and homologous cell-derived extracellular matrix (hcd-ECM) treated substratum differentially affected the above phenotypes. 3D MPM spheroid invasion was higher in FN-collagen matrices in the epithelioid and biphasic cells, while 3D cell cultures of epithelioid and sarcomatoid MPM cells in FN-collagen showed a higher contractility compared to hcd-ECM-collagen. Cell aggregates demonstrated invasive behaviour in hcd-ECM matrices alone. Our results suggest that ECM and the dimensionality affect malignant cell behaviour during cell culture studies. |
| Databáze: | OpenAIRE |
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