Genotoxicity induced by widely used pesticide binary mixtures on Caiman latirostris (broad-snouted caiman)

Autor: Evelyn Cecilia López González, Gisela Laura Poletta, Pablo Ariel Siroski
Rok vydání: 2019
Předmět:
Environmental Engineering
Erythrocytes
Health
Toxicology and Mutagenesis
0208 environmental biotechnology
Argentina
Glycine
Zoology
GLYPHOSATE
02 engineering and technology
INTERACTIONS
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Cypermethrin
chemistry.chemical_compound
CYPERMETHRIN
Otras Ciencias Veterinarias
Pyrethrins
medicine
Environmental Chemistry
Animals
Pesticides
Biochemical markers
0105 earth and related environmental sciences
Alligators and Crocodiles
Micronucleus Tests
biology
Ciencias Veterinarias
Public Health
Environmental and Occupational Health
General Medicine
General Chemistry
Pesticide
biology.organism_classification
Pollution
020801 environmental engineering
Caiman latirostris
CHLORPYRIFOS
chemistry
REPTILES
CIENCIAS AGRÍCOLAS
Chlorpyrifos
NUCLEAR ABNORMALITIES
Genotoxicity
DNA Damage
Mutagens
Zdroj: Chemosphere. 232
ISSN: 1879-1298
Popis: In this study, we investigated the effects of three binary mixtures of pesticide formulations commonly used in soybean crop: Cypermethrin Atanor® (25% -CYP), Chlorpyrifos Lorsban 48E® (48% -CPF) and Glyphosate Roundup® Full II (66.2% -GLY) on broad-snouted caiman (Caiman latirostris) hatchlings exposed by voluntary immersion under controlled condition. Genotoxicity was evaluated in peripheral blood erythrocytes through the micronucleus (MN) test and other nuclear abnormalities (NAs) and besides, growth of caiman was analyzed in each experimental group.The results showed that pesticide formulations tested, at concentrations similar to those recommended for application in the field, induced an increase in the frequency of micronucleus (FMN; p = 0.001) and Notched nuclei (NN; p = 0.010) in the mixture CYP + CPF, while an increase in the frequency of buds and NN was observed in the mixture of GLY + CYP (pbuds = 0.016 and pNN = 0.021), compared to the vehicle control (VC). On the contrary, a possible antagonistic action was observed between the components in the mixture GLY + CPF. Growth was not affected in any exposed groups (p > 0.05). There was a clutch effect in the frequency of binucleated erythrocytes (BiN; p = 0.011), total length (TL; p = 0.001) and snout-vent length (SVL; p = 0.031).Biomarkers used in this study are considered important predictive tools for the evaluation of xenobiotics. In this study, we demonstrated genotoxicity of pesticide mixtures under conditions that simulate the real situation of exposure suffered by caiman and other wild species in Argentina. Fil: López González, Evelyn Cecilia. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Poletta, Gisela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Databáze: OpenAIRE
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