Molecular Analysis of Carbon Ion-Induced Mutations in DNA Repair-Deficient Strains of Saccharomyces cerevisiae
| Autor: | Ayako N. Sakamoto, Katsuya Satoh, Yoshinobu Izumi, Kikuo Shimizu, Youichirou Matuo, Yoshihiro Hase |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Nuclear and High Energy Physics
DNA repair RAD52 Mutant Saccharomyces cerevisiae 8-oxodG Linear energy transfer Oxidative phosphorylation lcsh:Technology 030218 nuclear medicine & medical imaging 03 medical and health sciences S. cerevisiae 0302 clinical medicine Irradiation 030304 developmental biology high LET 0303 health sciences biology Chemistry lcsh:T food and beverages biology.organism_classification Molecular biology Quantitative Biology::Genomics Atomic and Molecular Physics and Optics MSH2 carbon ion beam lcsh:Electrical engineering. Electronics. Nuclear engineering mutation lcsh:TK1-9971 |
| Zdroj: | Quantum Beam Science, Vol 3, Iss 3, p 14 (2019) Quantum Beam Science Volume 3 Issue 3 |
| Popis: | Mutations caused by ion beams have been well-studied in plants, including ornamental flowers, rice, and algae. It has been shown that ion beams have several significantly interesting features, such as a high biological effect and unique mutation spectrum, which is in contrast to low linear energy transfer (LET) radiation such as gamma rays. In this study, the effects of double strand breaks and 8-oxo-2&prime deoxyguanosine (8-oxodG) caused by ion-beam irradiation were examined. We irradiated repair-gene-inactive strains rad52, ogg1, and msh2 using carbon ion beams, analyzed the lethality and mutagenicity, and characterized the mutations. High-LET carbon ion-beam radiation was found to cause oxidative base damage, such as 8-oxodG, which can lead to mutations. The present observations suggested that nucleotide incorporation of oxidative damage gave only a limited effect on cell viability and genome fidelity. The ion-beam mutations occurred predominantly in 5&prime ACA-3&prime sequences, and we detected a hotspot at around +79 to +98 in URA3 in wild-type and mutant lines, suggesting the presence of a mutation-susceptible region. |
| Databáze: | OpenAIRE |
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