Isolation and characterization of primary bone marrow mesenchymal stromal cells
Autor: | Hongzhe Li, Stefan Scheding, Roshanak Ghazanfari, Dimitra Zacharaki, Hooi Ching Lim |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Stromal cell Cellular differentiation Population Bone Marrow Cells Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice History and Philosophy of Science Genetic model medicine Animals Humans Progenitor cell education education.field_of_study General Neuroscience Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell biology Adult Stem Cells 030104 developmental biology medicine.anatomical_structure Phenotype Bone marrow Biomarkers Adult stem cell |
Zdroj: | Annals of the New York Academy of Sciences. 1370(1) |
ISSN: | 1749-6632 |
Popis: | Bone marrow (BM) contains a rare population of mesenchymal stromal cells (MSCs), which have been characterized as nonhematopoietic skeletal progenitor cells with central importance for the hematopoietic microenvironment. Classically, MSCs are isolated by plastic adherence and subsequent culture. However, as cultured stromal cells differ from their in vivo progenitors, it is important to identify the phenotype of the primary MSCs to study these cells in more detail. In the past years, several surface markers have been reported to be suitable for effective enrichment of BM-MSCs, and recent data indicate that the putative MSC stem/progenitor cell population in human adult BM is highly enriched in Lin(-) CD45(-) CD271(+) CD140a (PDGFRα)(low/-) cells. Moreover, surface marker combinations have been described for the isolation of MSCs from murine BM. On the basis of these findings, the role of primary MSCs can now be studied in normal and, importantly, diseased BM. Furthermore, genetically engineered mouse models have been developed as powerful tools to investigate well-defined BM stromal cell populations in vivo. Our discussion aims to provide a concise overview of the current state of the art in BM-MSC isolation in humans and briefly present murine MSC isolation approaches and genetic models. |
Databáze: | OpenAIRE |
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