Attempts to obtain fully xenogeneic fetuses in rat ↔ mouse model†,‡
| Autor: | Aneta Suwińska, Andrzej K. Tarkowski, Bohdan Paterczyk, Monika Humięcka, Marcin Szpila, Marek Maleszewski, Katarzyna Bożyk |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Uterus Embryonic Development Live fetus Biology Andrology Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Embryo Implantation Blastocyst reproductive and urinary physiology Fetus Chimera Embryo Cell Biology General Medicine Embryo Transfer Embryonic stem cell Rats 030104 developmental biology medicine.anatomical_structure Reproductive Medicine embryonic structures Rat uterus Female 030217 neurology & neurosurgery |
| Zdroj: | Biology of Reproduction. 102:499-510 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | The full-term development of the xenogeneic embryo in the uterus of the mother of different species is very restricted and can occur only in certain groups of closely related mammals. In the case of mouse ↔ rat chimeras, the interspecific uterine barrier is less hostile to interspecific chimeric fetuses. In current work, we tested the development of mouse and rat fetuses in uteri of females of the opposite species. We created chimeric mouse ↔ rat blastocysts by injection of mouse embryonic stem cells (ESCs) into eight-cell rat embryos and rat ESCs into eight-cell mouse embryos. Chimeras were transferred to the foster mothers of the opposite species. Despite a huge number of transferred embryos (>1000 in total for both variants), only one live fetus derived solely from the mouse ESCs was isolated at E13.5 from the rat uterus. All other fetuses and newborns were chimeric or were built only from the cells of the recipient embryo. We examined the possible reason for such an outcome and found that the xenogeneic fetuses are eliminated at the perigastrulation stage of development. Thus, we conclude that in the rat ↔ mouse combination even when extraembryonic tissues of the chimeric embryo are composed solely of the cells of the same species as the female to which embryos are transferred, the full-term development of the pure xenogeneic fetus is very unlikely. |
| Databáze: | OpenAIRE |
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