Allelic variation of the Tas1r3 taste receptor gene affects sweet taste responsiveness and metabolism of glucose in F1 mouse hybrids
| Autor: | V. A. Zolotarev, Egor A. Sozontov, Julia Andreeva, Raisa Khropycheva, Ekaterina A. Lukina, V. O. Murovets |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Inbred Strains Social Sciences Hemizygosity Biochemistry Receptors G-Protein-Coupled Fats Mice 0302 clinical medicine TAS1R3 Endocrinology Inbred strain Glucose Metabolism Taste receptor Genotype Medicine and Health Sciences Insulin Psychology Glucose Tolerance Genetics Mice Knockout Multidisciplinary Organic Compounds Monosaccharides Lipids Chemistry Phenotype Experimental Organism Systems Hemizygote Taste Physical Sciences Medicine Carbohydrate Metabolism Female Sensory Perception Research Article Science Carbohydrates Locus (genetics) Biology Research and Analysis Methods 03 medical and health sciences Food Preferences Animals Allele Alleles Diabetic Endocrinology Polymorphism Genetic Organic Chemistry Chemical Compounds Biology and Life Sciences Glucose Tolerance Test Hormones Mice Inbred C57BL 030104 developmental biology Glucose Metabolism Genetic Loci Animal Studies 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 7, p e0235913 (2020) |
| ISSN: | 1932-6203 |
| Popis: | In mammals, inter- and intraspecies differences in consumption of sweeteners largely depend on allelic variation of the Tas1r3 gene (locus Sac) encoding the T1R3 protein, a sweet taste receptor subunit. To assess the influence of Tas1r3 polymorphisms on feeding behavior and metabolism, we examined the phenotype of F1 male hybrids obtained from crosses between the following inbred mouse strains: females from 129SvPasCrl (129S2) bearing the recessive Tas1r3 allele and males from either C57BL/6J (B6), carrying the dominant allele, or the Tas1r3-gene knockout strain C57BL/6J-Tas1r3tm1Rfm (B6-Tas1r3-/-). The hybrids 129S2B6F1 and 129S2B6-Tas1r3-/-F1 had identical background genotypes and different sets of Tas1r3 alleles. The effect of Tas1r3 hemizygosity was analyzed by comparing the parental strain B6 (Tas1r3 homozygote) and hemizygous F1 hybrids B6 × B6-Tas1r3-/-. Data showed that, in 129S2B6-Tas1r3-/-F1 hybrids, the reduction of glucose tolerance, along with lower consumption of and lower preference for sweeteners during the initial licking responses, is due to expression of the recessive Tas1r3 allele. Hemizygosity of Tas1r3 did not influence these behavioral and metabolic traits. However, the loss of the functional Tas1r3 allele was associated with a small decline in the long-term intake and preference for sweeteners and reduction of plasma insulin and body, liver, and fat mass. |
| Databáze: | OpenAIRE |
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