A role for the immune system-released activating agent (ISRAA) in the ontogenetic development of brain astrocytes

Autor: Alyaa Mousa, Abdulaziz Isa AlJawder, Safa Taha, Moiz Bakhiet, Aminah M. I. Al-Awadi
Rok vydání: 2021
Předmět:
Macroglial Cells
Cell signaling
Physiology
Cellular differentiation
Cell
Stimulation
Signal transduction
Biochemistry
Tyrosine Kinases
chemistry.chemical_compound
Animal Cells
Immune Physiology
Medicine and Health Sciences
STAT1
Post-Translational Modification
Phosphorylation
Enzyme-Linked Immunoassays
Lymphokines
Mice
Inbred BALB C
Innate Immune System
Multidisciplinary
medicine.diagnostic_test
Brain
Cell Differentiation
Enzymes
Cell biology
STAT signaling
medicine.anatomical_structure
Medicine
Cytokines
Cellular Types
Tyrosine kinase
Research Article
Astrocyte
Science
Immunology
Glial Cells
Biology
Research and Analysis Methods
Antibodies
Interferon-gamma
Mediator
Immune system
Western blot
medicine
Animals
Immunoassays
Immunohistochemistry Techniques
Cell Proliferation
Biology and Life Sciences
Proteins
Tyrosine phosphorylation
Cell Biology
Molecular Development
Embryonic stem cell
Histochemistry and Cytochemistry Techniques
chemistry
Astrocytes
Immune System
biology.protein
Immunologic Techniques
Enzymology
Protein Kinases
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 5, p e0248455 (2021)
ISSN: 1932-6203
Popis: The Immune System-Released Activating Agent (ISRAA) was discovered as a novel molecule that functions as a mediator between the nervous and immune systems in response to a nervous stimulus following an immune challenge. This research investigated the role of ISRAA) in promoting the ontogeny of the mouse brain astrocytes. Astrocyte cultures were prepared from two-month-old BALB/c mice. Recombinant ISRAA protein was used to stimulate astrocyte cultures. Immunohistochemistry and ELISA were utilized to measure ISRAA and IFN-γ levels, IFN-γR expression and STAT1 nuclear translocation. MTT-assay was used to evaluate cellular survival and proliferation. To assess astrocyte cell lysates and tyrosine-phosphorylated proteins, SDS-PAGE and western blot were used. ISRAA was highly expressed in mouse embryonic astrocytes, depending on cell age. Astrocytes aged seven days (E7) showed increased proliferation and diminished differentiation, while 21-day-old (E21) astrocytes depicted reversed effects. ISRAA stimulated the tyrosine phosphorylation of numerous cellular proteins and the nuclear translocation of STAT1. IFN-γ was involved in the ISRAA action as ISRAA induced IFN-γin both age groups, but only E21 astrocytes expressed IFN-γR. The results suggest that ISRAA is involved in mouse brain development through the cytokine network involving IFN-γ.
Databáze: OpenAIRE
Externí odkaz: