Characterization of biocide-tolerant bacteria isolated from cheese and dairy small-medium enterprises
Autor: | Rubén Pérez Pulido, Ma Luisa Fernández Márquez, Mª Carmen López Aguayo, Ma José Grande Burgos, Rosario Lucas, Antonio Gálvez |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Biocide Tetracycline medicine.drug_class 030106 microbiology Antibiotics Microbial Sensitivity Tests Microbiology Antiporters 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance Bacterial Proteins Enterobacteriaceae Cheese Drug Resistance Bacterial medicine Animals Bacteria biology Escherichia coli Proteins Sequence Analysis DNA Enterobacter biology.organism_classification Triclosan Anti-Bacterial Agents Dairying Milk 030104 developmental biology chemistry Lactobacillaceae Food Microbiology Cattle Female Efflux Benzalkonium Compounds Disinfectants Food Science medicine.drug |
Zdroj: | Food Microbiology. 62:77-81 |
ISSN: | 0740-0020 |
Popis: | A collection of 120 bacterial isolates from small medium enterprises involved in the production of cow milk and the manufacture of goat cheese were screened for sensitivity to biocides benzalkonium chloride (BC), cetrimide (CT), hexadecylpyridinium chloride (HDP), triclosan (TC), hexachlorophene (CF) and poly-(hexamethylen guanidinium) hydrochloride (PHMG). Nineteen isolates were selected according to biocide tolerance and identified by 16S rDNA sequencing as Lactococcus sp. (6) Enterococcus sp. (1), Lactobacillus sp. (4), Bacillus sp. (1) Escherichia sp. (5), Enterobacter sp. (1) and Helicobacter sp. (1). These were further characterised regarding antimicrobial resistance phenotype and genotype. Several isolates were multiply (3 or more) tolerant to biocides or resistant to antibiotics, but only two Escherichia sp. isolates and Enterobacter sp. were multiply resistant to biocides and antibiotics. Statistical analysis of biocide tolerance and antibiotic resistance revealed significant positive correlations between different biocides and between biocides and antibiotics. The biocide tolerance genes most frequently found were qacEΔ1 and qacA/B. The sulfonamide resistance gene sul1 was found in two Escherichia sp. isolates and in Enterobacter sp., all of which also carried qacEΔ1. Beta-lactam (blaCTX-M, blaPSE) and tetracycline resistance genes [tet(A), tet(C) and tet(D)] were detected. Efflux pump genes acrB and mdfA were found in most Gram-negative isolates. Results from the study suggest that exposure to biocides can indirectly select for antibiotic resistance. |
Databáze: | OpenAIRE |
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