Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae (Danio rerio)

Autor: Rosane Souza da Silva, Fabiano Peres Menezes, Luiza Reali Nazario, Luiza Wilges Kist, Lidiane Fazenda, Angela T. S. Wyse, Maurício Reis Bogo, Katiucia Marques Capiotti, Carla Denise Bonan
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Arginine
Adenosine Triphosphate/metabolism
Developmental/drug effects
Larva/drug effects
Adenosine Triphosphate
Nucleotidases
Ectonucleotidase
Adenosine Monophosphate/metabolism
Zebrafish
Adenosine Diphosphate/metabolism
Brain/drug effects
biology
Purinergic receptor
Age Factors
Brain
Gene Expression Regulation
Developmental
Purinergic signalling
Adenosine Diphosphate
Larva
Nucleotidases/metabolism
Drug
medicine.medical_specialty
animal structures
Motor Activity/drug effects
Danio
Gene Expression Regulation
Enzymologic/drug effects
Motor Activity
Gene Expression Regulation
Enzymologic
Dose-Response Relationship
Developmental Neuroscience
Internal medicine
medicine
Extracellular
Animals
Analysis of Variance
Gene Expression Regulation
Developmental/drug effects
Dose-Response Relationship
Drug
Cell Membrane/drug effects
Cell Membrane
Enzymologic/drug effects
biology.organism_classification
medicine.disease
Molecular biology
Adenosine Monophosphate
Endocrinology
Gene Expression Regulation
Inborn error of metabolism
Arginine/pharmacology
Developmental Biology
Zdroj: International Journal of Developmental Neuroscience, 31(1), 75-81. Elsevier-Pergamon
Repositório Institucional PUCRS
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
ISSN: 0736-5748
DOI: 10.1016/j.ijdevneu.2012.09.002
Popis: Hyperargininemia is an inborn error of metabolism (IEM) characterized by tissue accumulation of arginine (Arg). Mental retardation and other neurological features are common symptoms in hyperargininemic patients. Considering purinergic signaling has a crucial role from the early stages of development and underlying mechanisms of this disease are poorly established, we investigated the effect of Arg administration on locomotor activity, morphological alterations, and extracellular nucleotide hydrolysis in larvae and adult zebrafish. We showed that 0.1 mM Arg was unable to promote changes in locomotor activity. In addition, 7-day-post-fertilization (dpf) larvae treated with Arg demonstrated a decreased body size. Arg exposure (0.1 mM) promoted an increase in ATP, ADP, and AMP hydrolysis when compared to control group. These findings demonstrated that Arg might affect morphological parameters and ectonucleotidase activities in zebrafish larvae, suggesting that purinergic system is a target for neurotoxic effects induced by Arg.
Databáze: OpenAIRE
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