Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici
| Autor: | William E. Michener, Xiaoqing Wang, Violeta Sànchez i Nogué, John R. Dorgan, Davinia Salvachúa, Gregg T. Beckham, Adam D. Bratis |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biochemicals lcsh:Biotechnology Biomass Lignocellulosic biomass Management Monitoring Policy and Law 01 natural sciences Applied Microbiology and Biotechnology Hydrolysate lcsh:Fuel 03 medical and health sciences chemistry.chemical_compound lcsh:TP315-360 010608 biotechnology lcsh:TP248.13-248.65 Organic acids Yeast extract Food science Renewable Energy Sustainability and the Environment Chemistry Research Lactic acid Biorefinery 030104 developmental biology General Energy Corn stover Agronomy Biofuel Fermentation Biotechnology |
| Zdroj: | Biotechnology for Biofuels Biotechnology for Biofuels, Vol 10, Iss 1, Pp 1-13 (2017) |
| ISSN: | 1754-6834 |
| Popis: | Background The production of value-added chemicals alongside biofuels from lignocellulosic hydrolysates is critical for developing economically viable biorefineries. Here, the production of propionic acid (PA), a potential building block for C3-based chemicals, from corn stover hydrolysate is investigated using the native PA-producing bacterium Propionibacterium acidipropionici. Results A wide range of culture conditions and process parameters were examined and experimentally optimized to maximize titer, rate, and yield of PA. The effect of gas sparging during fermentation was first examined, and N2 was found to exhibit improved performance over CO2. Subsequently, the effects of different hydrolysate concentrations, nitrogen sources, and neutralization agents were investigated. One of the best combinations found during batch experiments used yeast extract (YE) as the primary nitrogen source and NH4OH for pH control. This combination enabled PA titers of 30.8 g/L with a productivity of 0.40 g/L h from 76.8 g/L biomass sugars, while successfully minimizing lactic acid production. Due to the economic significance of downstream separations, increasing titers using fed-batch fermentation was examined by changing both feeding media and strategy. Continuous feeding of hydrolysate was found to be superior to pulsed feeding and combined with high YE concentrations increased PA titers to 62.7 g/L and improved the simultaneous utilization of different biomass sugars. Additionally, applying high YE supplementation maintains the lactic acid concentration below 4 g/L for the duration of the fermentation. Finally, with the aim of increasing productivity, high cell density fed-batch fermentations were conducted. PA titers increased to 64.7 g/L with a productivity of 2.35 g/L h for the batch stage and 0.77 g/L h for the overall process. Conclusion These results highlight the importance of media and fermentation strategy to improve PA production. Overall, this work demonstrates the feasibility of producing PA from corn stover hydrolysate. |
| Databáze: | OpenAIRE |
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